摘要
目的研究神经生长抑制因子Nogo-A mRNA在缺氧缺血性脑损伤（hypoxic-ischemic brain damage，HIBD）新生大鼠脑组织的动态变化，探讨Nogo-A基因对HIBD新生大鼠中枢神经再生的影响。方法将180只SD新生大鼠随机分为对照组（n=90）和实验组（n=90），实验组制成HIBD模型，根据处死动物的时间不同随机分为HIBD后3、7、14、21和28d共5个亚组，每组18只；对照组也在相应时间点取材；采用原位杂交法检测新生大鼠脑组织Nogo-A mRNA在不同时间的表达量。结果原位杂交法显示Nogo-A mRNA在实验组新生大鼠的皮层呈强阳性表达，阳性细胞分布密集，对照组阳性细胞分布较实验组稀疏；实验组新生大鼠脑组织Nogo-A mRNA阳性细胞数量在缺血缺氧后7d（34．73±3．34）比14d（36．67±5．33）和21d（42．33±2．21）均较对照组7d（28．67±2．91）、14d（32．17±5．06）和21d（36．83±4．90）明显增高（P〈0．05或0．01）。结论脑组织Nogo-A mRNA表达在脑损伤急性期（3d）变化不明显，而在1周后较为明显，提示缺氧缺血性脑损伤后期Nogo-A mRNA表达明显升高，它可能是造成新生大鼠脑损伤后神经再生障碍的重要原因之一。
Objective To study the changes of nerve growth inhibitor Nogo-A mRNA in the brain of newborn rats with hypoxic-ischemic brain damage （HIBD） and its effects on regeneration of central nervous system. Methods One hundred and eighty newborn rats were divided into experiment group （n=90） and control group （n=90）. Newborn rats in experiment group were established HIBD model Situ-hybridization was used to detect the expression of Nogo-A mRNA in the brain of all rats at different time after HIBD or birth. Results Situ-hybridization showed that, Nogo-A positive cells in cerebral cortex in experiment group were stronger and densely covered than in control group. In experiment group, the number of Nogo-A positive cells at cerebral cortex at 7 days after hypoxic and ischemic （34.73±3.34）, 14 days （36.67±5.33）, and 21 days （42.33±2.21） were more than that at 7 days （28.67±2.91）, 14 days （32.17±5.06） and 21 days （36.83±4.90） in control group. There was no statistical difference between 21^th day and 28^th day （P〉0.05）. Comparing with control group, the number of Nogo-A positive cells in experiment group at 3, 7, 14 and 21 days were higher （P〈0. 05）. Conclusions The expression of Nogo-A mRNA in cerebral cortex changed slightly at the first period（3d） after hypoxic and ischemic but obviously after one week. Which indicated that dramatically increased Nogo-A mRNA in the latter period after hypoxic and ischemic may be one of the important causes of the central nervous system regeneration obstacle in the HIBD newborn rats.
出处
《中华围产医学杂志》
CAS
2007年第1期37-40,73,共5页
Chinese Journal of Perinatal Medicine
基金
广东省医学科学技术研究基金资助项目（A2004305）
