摘要
构建了A3启动子缺陷piggyBac转座质粒,以增强型绿色荧光蛋白基因EGFP为标记基因对家蚕品种N is-tari进行转基因实验,发现其具有较高的表达EGFP的转化效率,G0代中EGFP阳性蛾区检出占总注射蚕卵的比率达0.618%,且EGFP的表达呈组织特异性。PCR实验分析也证实了标记基因的成功导入。该实验中标记基因及转基因阳性个体均易于检出,为启动子缺陷转基因方法在家蚕组织特异性启动子筛选研究上的应用奠定了基础。
We constructed the plasmid harbored the A3 promoter-deficient piggyBac-derived transposon and the enhanced green fluorescence protein （EGFP） gene. The constructed plasmid was microinjected into the eggs of Nistari strain, along with the helper plasmid. Transgenic experiment results showed that the transformation efficiency was high, and the rate of number of GO moths whose offspring expressed the EGFP to the number of eggs injected was 0. 618%. Moreover, EGFP was expressed with the manner of specific tissue, and the positive moths are easy to be observed in Go generation. The transgenic silkworms were also verified by PCR method. Our results indicated that A3 promoter-deficient transformation in the silkworm maybe used to screen the promoter activated in specific tissue.
出处
《蚕业科学》
CAS
CSCD
北大核心
2008年第1期41-44,共4页
ACTA SERICOLOGICA SINICA
基金
国家高技术研究发展计划“863”项目(编号2006AA10A-118)
国家重点基础研究发展计划“973”项目(编号200-5CB121003)
博士点基金项目(编号20070335148)
浙江省科技厅重点科研项目(编号2005C22046)
