期刊文献+

哺乳动物转基因载体——piggyBac转座子

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摘要 piggyBac转座子具有识别位点特异性5'-TTAA-3',剪切和插入都不留下印迹,且高效转座。基于piggyBac转座子的特性,借助载体系统,已应用于哺乳动物转基因的研究。此外,piggyBac转座子还应用于基因诱导突变和基因治疗等领域。RNAi技术作为基因功能研究的一个工具,在应用于克服畜牧业中家畜的生产繁殖障碍等方面已取得一定研究成果。本文对piggyBac转座子的结构和特性、转座机制及影响因素和在动物中应用等方面进行总结,为RNAi技术借助piggyBac转座子用于哺乳动物转基因提供理论依据。
出处 《畜牧与兽医》 北大核心 2012年第5期92-95,共4页 Animal Husbandry & Veterinary Medicine
基金 农业部948项目(2010-Z16) 转基因专项(2009ZX08009-142B) 湖北省和武汉市国际合作项目(YJH0044 201070934339 201171034317)
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参考文献12

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