摘要
应用均匀设计方法对香石竹组培苗的增殖体系进行优化研究,设计BA浓度、琼脂用量、蔗糖浓度、通气孔大小和氨态氮浓度5个因素试验,对观察值进行方差分析,结果表明:MS+BA 0.1 mg/L+琼脂7.5 g/L+蔗糖35 g/L+铵态氮10 mM处理相对较好,通气口大小2.25 cm,通过10个处理的回归分析得出5个因素对香石竹试管苗生长作用中不同调查项目4个拟合回归方程,并计算出极值和最佳因素配比,发现BA不适宜用在香石竹的增殖培养.
The optimization of plant regeneration system in vitro culture of Dianthus caryophyllus L. was studied with the uniform design. The experiment optimized by uniform design experiment of 5 factors at 10 concentration levels. The 5 factors include 6-BA, agar,sucrose,venthole and NH4^＋-N. The results indicated that NO. 7 is the best,the medium MS ＋ BA 0.1 mg/L ＋ agar 7.5 g/L ＋ sucrose 35 g/L ＋ NH^4＋- N 10 mM and venthole 2.25 cm. The 10 levels were treated by regression analysis,4 regression equations were obtained. According to the 4 equations, the maximum was calculated and the best allocated proportion was obtained, BA is not fit to multiplicate for Dianthus caryophyllus L.
出处
《延边大学农学学报》
2008年第1期65-68,共4页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金资助项目(30560094)
关键词
香石竹
组织培养
均匀设计
Dianthus caryoph yllus L.
tissue culture
uniform designing
