摘要
目的:观察吲哚美辛对SD大鼠实验性变态反应性脑脊髓炎的治疗效果,初步探讨其治疗机制。方法:采用同种脑脊髓匀浆诱导实验性变态反应性脑脊髓炎模型,给予吲哚美辛进行实验性干预治疗,观察和比较动物的神经症状和病理改变。用免疫组化法观察各组动物小胶质细胞反应。结果:吲哚美辛可以缓解临床症状、缩短病程、降低实验性变态反应性脑脊髓炎的缓解率、复发率,减轻炎症反应及脱髓鞘(P<0.05)。治疗组大鼠脑组织中小胶质细胞的阳性细胞数在高峰期和未治疗组相比,有统计学意义。结论:吲哚美辛抑制小胶质细胞活性,下调细胞因子TNF-α的水平;从而对实验性变态反应性脑脊髓炎具有保护作用。
Objective To explore the therapeutic effect of indomethacin on experimental allergic encephalomyelitis model in SD rat. Methods The animal model was established in SD rat by injecting isogenic immunogen in Freud＇s complete adjuvant and bordetella pertussis vaccine. Experimental treatment was given by using indomethacin, then its clinical symptoms and histological alteration were observed and compared. The microglia reactions to experimental allergic encephalomyelitis lesions were examined in each group at various stages with the method of immunohistochemistry and ELISA. Results Indomethacin could inhibit the decrease of experimental allergic encephalomyelitis rats＇ weight, alleviate clinical symptom, shorten disease course, reduce incidence of relapse, lighten inflammatory reaction and demyelinatlon（P〈0. 05）. The number of brain microglia at the peak of disease was significant between treatment group and no treatment group. Conclusion Indomethacin could inhibit activation of microglla, depress the expression of TNF-α, and protect experimental allergic encephalomyelitis rat.
出处
《实用诊断与治疗杂志》
2008年第4期246-248,I0001,共4页
Journal of Practical Diagnosis and Therapy
基金
广东省科技计划项目(2006B36004006)
广州市科技计划项目(2006J1-C0231)
广州市属高校科技项目(61022)
番禺区科技计划项目(2006-Z-44-1)
