摘要
参照已发表的H5N1亚型禽流感的HA基因序列，设计了1对引物，对禽流感病毒新疆株A／Duck／XJ／3（H5N1）、A／Duck／XJ／4（H5N1）、A／CH／XJ／5（H5N1）、A／Goose／XJ／10（H5N1）和A／Duck／XJ／11（H5N1）进行RT—PCR扩增和克隆，获得了5个毒株的HA全长核苷酸序列，分别为1779bp、1774bp、1776bp、1758bp和1740bp序列分析表明，在HA切割位点处均有多个连续的碱性氨基酸（-R-E-R-R-R-K-K-R-）插入，具有高致病性禽流感病毒的特征，同源性分析表明，新疆分离株之间的核苷酸同源性在96.9％～98.8％；与来自青海湖斑头雁、广西天鹅、香港白鹭和台湾鸽等不同野禽毒株同源性为95.7％～100％；与来自香港、越南、浙江的人源禽流感毒株的同源性为95.2％～98.2％。
According to the published HA gene sequence of H5N1 subtype of avian influenza virus （AIV）, a pair of primers was designed. The HA gene of A/Duck/XJ/3 （H5N1）, A/Duck/XJ/4 （H5N1）, A/ CH/XJ/5（H5N1）, A/Goose/XJ/10（H5N1） and A/Duck/XJ/11（H5N1） were amplified by RT-PCR and cloned. The HA complete nucleotide sequences of each strain were 1 779 bp, 1 774 bp,1 776 bp,1 758 bp and 1 740 bp respectively. The sequences analysis indicated that there were serial basic amino acids （-R-E-R-R-R-K-K-R-） at the cleavage site of HA, which was consistent with the characteristics of highly patho- genic AIV. The sequences analysis showed that the nucleotide homology among Xinjiang AIV strains was 96. 9% ～ 98. 8%. Comparitive analysis of Xinjiang AIV strains with those from Qinghai, Guangxi, Hongkong and Tailand bird flu indicated that the nucleotide homology was 95.7%～100 %. The nucleotide homology of Xinjiang AIV strains in comparison with the human AIV isolates from Hongkong, Vietnam and Zhejiang was 95.2%～98.2%.
出处
《中国兽医杂志》
CAS
北大核心
2008年第3期3-5,共3页
Chinese Journal of Veterinary Medicine
基金
新疆自治区科技厅重点项目(200431101)
