摘要
用RT-PCR法，以1株H5N1亚型禽流感病毒新疆分离株A／Duck／XJ／4为模板，扩增了NP全基因cD-NA，克隆至T载体，并进行序列测定。序列分析结果表明：扩增的NP基因全长1518 bp，最大的开放阅读框在18～1514 bp，编码499个氨基酸。将A／Duck／XJ／4毒株NP基因序列与15株A型流感病毒NP基因序列进行比较，各毒株间NP基因核苷酸序列同源性81．4％～99．0％，编码的氨基酸同源性在92．8％～99．6％。
NP gene of A/Duck/XJ/4 strain of N5H1 avian influenza virus was used as template. The cDNA of NP gene was amplified by RT-PCR method and was cloned into T vector and then was sequenced. The cloned 1 518 bp fragment covered open reading frame of NP gene and encoded 499 amino acids. The results of sequence analysis indicated that the nucleotide sequence of NP gene of A/Duck/XJ/4 strain shared 81.4%--99.0% homology with other fifteen isolates and encoded amino acid sequence shared 92.8%--99.6% homology with other fifteen isolates.
出处
《新疆农业大学学报》
CAS
2008年第3期9-12,共4页
Journal of Xinjiang Agricultural University
基金
新疆自治区科技厅重点项目(00431101)
