摘要
目的探讨大黄对严重烧伤大鼠肠黏膜屏障的保护作用。方法将36只SD大鼠随机分为对照组(n=6)、烧伤组(n=15)和大黄治疗组(n=15)。对照组不作任何处理;烧伤组和大黄治疗组均于背部造成30%TBSAⅢ度烫伤(以下称烧伤)创面,伤后立即经腹腔补充乳酸林格液(40 mL/kg),大黄治疗组大鼠伤后通过灌胃给予大黄混悬液(60 mg/kg)治疗。检测各组大鼠伤后72 h的血浆内毒素含量,取肠系膜淋巴结(MLN)、肝、脾组织匀浆后进行细菌培养,对空肠黏膜组织进行病理形态学分析。结果血浆内毒素含量大黄治疗组<对照组<烧伤组;肠系膜淋巴结、肝、脾组织匀浆细菌移位量大黄治疗组<烧伤组;空肠黏膜组织病理形态学观察发现,大黄治疗组较烧伤组绒毛上皮细胞水肿减轻,绒毛被覆上皮比较完整,绒毛顶端上皮细胞坏死、脱落减少。结论大黄能减轻严重烧伤引起的肠黏膜损伤,可减少肠源性感染的发生。
Objective To explore the protective effect of rhubarb on intestinal mucosal barrier in rats with severe burns. Methods The rats were randomly divided into control group （n = 6, without treatment）, burn group（n = 15, with 30 % TBSA full-thickness burn on the back）and rhubarb group（n=15,fed with rhubarb after burns）. The rats in the groups with burn injury were resuscitated with intraperitoneal isotonic saline after burned. At 72 hours after burned,the concentration of endotoxin in plasma of all rats was detected and the mesenteric lymphatic nodes （MLN）, liver and spleen were homogenized to culture for bacteria. Finally, the sections of jejunum tissues were routinely prepared and examined. Results The concentration of plasma endotoxin in rhubarb group〈in control group〈in burn group. Bacterial translocation quantity in MLN,liver, and spleen in rhubarb group〈in burn group. Histological examination showed,in rhubarb group the pathomorphologic injury in the intestinal mucosa was slighter than those in burn group. Con- clusion Rhubarb could decrease the intestinal mucosal damage induced by burn, and thereby could keep the defense function of intestinal mucosal barrier against bacteria and endotoxin.
出处
《江西医学院学报》
CAS
2008年第1期16-18,F0004,共4页
Acta Academiae Medicinae Jiangxi
关键词
大黄
烧伤
肠黏膜屏障
动物
实验
大鼠
rhubarb
burn
intestinal mucosal barrier
animal, experiment
rat
