摘要
目的:研究昆布提取物(TLPE)在体外对耐药细胞B-MD-C1(ADR+/+)的逆转作用,探讨其逆转肿瘤多药耐药的机制.方法:应用流式细胞术测定不同剂量TLPE对B-MD-C1用药前后细胞内蛋白激酶C-α(PKC-α)和谷胱甘肽转移酶-π(GST-π)的表达变化,测定细胞周期和凋亡率;应用姬姆萨染色技术观察细胞形态学改变及细胞凋亡情况;应用比色法检测各组细胞ATP酶(ATPase)变化.结果:TLPE在70~300mg/L浓度范围内能明显降低B-MD-C1细胞内PKC-α和GST-π的表达(P<0.01),并将细胞阻滞于S期,但不诱导细胞凋亡;TLPE对细胞内ATPase无影响(P>0.05).结论:TLPE对子宫内膜癌细胞系B-MD-C1(ADR+/+)具有耐药逆转作用,其逆转作用与其诱导PKC-α和GST-π的表达降低有关.
AIM： To study the multidrug resistance （MDR）- reversing effect of Chinese drug Thallus Larninariae PE （ TLPE ） on human endometrial cancer cell line B-MD-C1 （ADR^＋/＋ ） in vitro and its underlying mechanism. METHODS： The expressions of PKC-α and GST-π at the level of protein in B-MD-C1 cell treated with different doses of TLPE were analyzed by flow cytometry. The cell cycle and apoptosis rate of B-MD-C1 （ADR＋/＋ ） cells treated by TLPE were detected. Their morphological changes were observed by Gimsa staining methods. Colorimetry was used to detect the changes of ATPase on B-MD-C1 （ADR^＋/＋ ） cells treated by TLPE. RESULTS： After interfered by TLPE （70 - 300mg/L）, the expressions of PKC-α and GST-π in cells were decreased significantly （ P 〈 0.01 ）. TLPE could block cell cycle at S period, but it did not obviously induce cell apoptosis. There was no significant change in the expression of ATPase on TLPE- treated cells. CONCLUSION： TLPE can reverse MDR of B-MD-C1 （ADR^＋/＋ ） cell, and the mechanism may be related to the decreasing expressions of PKC-α and GST-π in cells.
出处
《第四军医大学学报》
北大核心
2008年第5期474-476,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30371753)
