摘要
目的 探讨 5种多药耐药 (MDR)基因在胃癌组织中的表达 ,建立 MDR的基因诊断标准 .方法 用逆转录 -聚合酶链反应法 (RT- PCR)及流式细胞术检测胃癌组织中 5种MDR基因的 m RNA和蛋白质的表达 ,用四氮唑蓝快速比色法 (MTT)检测抗癌药物对胃癌原代细胞的 IC50 值 .结果 胃癌组织 mdr1,MRP,L RP和 GSTP1 m RNA表达增加 ,Topo α m RNA表达量减少 ;5种 MDR基因 m RNA表达量之间无相关性 ;胃癌耐药涉及 m dr1,MRP,L RP,GSTP1 和 Topo α基因 .mdr1m RNA≥ 0 .4 ,MRP m RNA≥ 0 .5 ,L RP m RNA≥0 .9,GSTP1 m RNA≥ 0 .8和 Topo α m RNA≤ 0 .3为耐药联合诊断标准 ,符合率为 98.6 % .结论 胃癌组织中存在 5种MDR基因 ,分别介导不同的多药耐药现象 ,多重 MDR是胃癌耐药的主要形式 .用 RT- PCR方法联合检测 5种 MDR基因 m RNA表达在胃癌化疗敏感性预测中具有必要性和可行性 .
AIM To explore the expression of five multiple drug resistance (MDR) genes in gastric carcinoma, and establish the diagnostic standard for MDR genes. METHODS RT PCR and flow cytometry (FCM) were performed to detect the expression of mRNA and protein in five MDR genes,and MTT method was used to test the IC 50 of different anti cancer reagent in primary cultured gastric cancer. RESULTS The mRNA expression of mdr1, MRP, LRP, GST P1 were increased, and TopoⅡαmRNA was decreased; there was no significant correlation between five MDR genes expression by detecting their mRNA amounts; mdr1, MRP, LRP, GST P1 and TopoⅡα genes were involved in the drug resistance of gastric cancer. Combined mdr1 mRNA≥0.4, MRP mRNA≥0.5, LRP mRNA ≥0.9, GST P1 mRNA≥0.8 and TopoⅡα mRNA≤0.3 were the diagnostic standard for drug resistance. The coincidence rate was 98.60%. CONCLUSION It was suggested that five MDR genes exist in gastric carcinoma, and the five genes mediate different drug resistance separately. It is necessary and practical to detect the five MDR genes expression by using RT PCR in order to set an individual scheme of chemotherapy.
出处
《第四军医大学学报》
CAS
北大核心
2002年第21期1952-1954,共3页
Journal of the Fourth Military Medical University
