摘要
采用工业化常用的高压匀浆破碎法对螺旋藻细胞破碎释放藻蛋白的过程进行系统研究。考察了匀浆次数、操作压力、悬浮体系、进料藻细胞密度等对螺旋藻细胞破碎率的影响。优化得到的高压均浆破碎螺旋藻细胞的工艺为:操作压力为80MPa,悬浮体系为pH6.810mmol/L磷酸盐缓冲液,匀浆破碎次数为三次。螺旋藻细胞破碎率为86%,此时可获得的粗藻蛋白总含量为73%。本研究还对高压匀浆破碎螺旋藻过程的动力学进行了分析。
The fragmentation of spintlina cells by a high-pressure homogenizer was investigated for the extraction of proteins in spirulina cells. A systematic study was carried out on the influence of physical and chemical factors including homogenizer passes, operating pressure and suspension system during homogenization processes. Under the optimal conditions： 80 MPa, pH6.8 10 mmol/L phosphate buffer suspending system, 3 times passing through the homogenizer, the spirulina cells fragmentation efficiency could reach 86%, and the total protein content released by spirulina cells was 73%. Meanwhile, kinetic studies of this disruption process had been made, a kinetic model was used and kinetic parameters were obtained under different conditions.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第3期43-45,共3页
Food Science
基金
天津市科技攻关计划重点专项项目(06YEGZNC04200)
关键词
螺旋藻
高压匀浆
细胞破碎
总蛋白含量
spirulina
high pressure homogenizer
cell fragmentation
total protein content
