摘要
目的:研究β-细辛醚对慢性点燃癫痫大鼠脑组织即早基因c-fos表达的影响。方法:大鼠随机分为β-细辛醚高、中、低(200,100,50mg·kg-1.d-1)剂量组,阳性药对照组(苯妥英钠36mg·kg-1),模型对照组(基质),灌胃给药。以青霉素点燃大鼠慢性癫痫,采用westernblot法测定海马c-fos的表达,免疫组化法测定脑皮质c-fos的表达。结果:β-细辛醚能显著增强癫痫大鼠脑内c-fos的表达,且呈量效关系。海马c-fos的表达量:β-细辛醚高、中、低剂量组为(1139.45±155.56),(1109.56±134.03),(1103.73±235.82)CNT.mm2,基质对照组(920.54±203.20)CNT.mm-2,苯妥英钠组(1106.26±186.24)CNT.mm2。皮质c-fos阳性细胞数:β-细辛醚高、中、低剂量组为(87.1±2.2),(76.3±1.3),(59.9±1.3)个,基质对照组(39.3±2.6)个,苯妥英钠组(95.2±1.1)个。结论:β-细辛醚能增强癫痫大鼠脑内c-fos的表达,是机体重要的抗癫痫反应。
Objective： To study the effects of β-asarone on expression of immediately early gene c-fos in kindling epilepsy rat brain. Method： The rats were randomly divided in to β-asarone groups （200, 100, 50 mg · kg^-1 · d^-1）, difetoin control group （36 nag · kg^-1 ） and model group. The remedy was administered orally. The effects were observed in kindling epilepsy model induced by penicillin, then the expression of c-fos were determined by western blot （hippocampus） and immunohistochemical techniques （ cortex）. Result： β-asarone could significantly increase the expression of c-fos in kindling epilepsy rat brain, and show its quantity-effect relation. The expression of c-fos in hippocampus was （ 1 139.45 ±155.56）, （ 1 109. 56 ± 134. 03 ）, （ 1 103.73 ± 235.82） CNT · mm^2 in β-asarone groups, 920. 54 ±203. 20 in model control group, and 1 106. 26 ± 186. 24 in difetoin group, respectively. The number of c-fos positive cell was 87. 1 ± 2. 2,76.3 ± 1.3 and 59. 9 ± 1.3 in β-asarone groups, 39. 3 ± 2. 6 in model control group, and 95.2 ± 1.1 in difetoin group, respectively. Conclusion： β-asarone can obviously increase the expression of c-fos in epilepsy rat brain. It is one of important response to epilepsy.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第5期534-536,共3页
China Journal of Chinese Materia Medica
