摘要
为了探讨神经-免疫-内分泌网络中生物活性物质间相互调节和平衡的关系,本试验应用免疫组化双标记法对成年SD雌性大鼠下丘脑中IFN-γ受体(IFN-γR)和雌激素受体(ER)的共表达进行了研究。结果发现,IFN-γR和ER广泛存在于大鼠下丘脑中,其中在视前交叉上核、视前内侧核、室周核、交叉上核、下丘脑前核、下丘脑内侧核、下丘脑外侧核、乳头体前背侧核、乳头体前腹侧核等13个核团中存在大量的IFN-γR和ER双标记细胞,双标记细胞约占全部阳性标记细胞的60.9%;双标记细胞胞质呈黑褐色、胞核呈棕黄色;ER单标着色细胞以神经胶质细胞居多。研究结果表明,IFN-γ和雌激素可以分别以其各自受体为介质进行信号传递和信息整合,同时也通过在同一细胞中的相互作用而参与机体的神经-免疫-内分泌调节。
In order to explore the interaction and balance relationship between substances in the neuro-endocrine-immunoregulatory network, we researched the coexistence of the IFN-γR and ER immunoreactivity（ir） in the hypothalamus of healthy adult female SD rat by immunohistochemistry method. The results were as follows： there were IFN-γR-ir and ER-ir single and double labeled cells in the hypothalamus. The double labeled cells were mainly located in the suprachiasmatic nucleus, anteromedial nucleus, anterolateral nucleus, periventricular nucleus, supraoptic nucleus, periventricular nucleus, nuclei of anterior hypothalamic nucleus, poster hypothalamic nucleus, ventral premammillary nucleus, dorsa double labelled cells accounted for about 60. 9 remammillary nucleus, and so on. IFN-γR/ER of total labeled cells. The IFN-γR-ir producs were blue-black, which were mainly located in the cytoplasm. The ER-ir products were brown in color, which were mainly located in the whole neurons. These results implied that there were interaction between the IFN-γR and ER in the neuro-endocrine-immunoregulatory network, which probably join in the action of the neuro-endocrine-immunoregulatory network.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第2期218-222,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
西北农林科技大学科研启动基金
河南花花牛乳业基金
关键词
γ-干扰素受体
雌激素受体
免疫组织化学法
共存
下丘脑
大鼠
interferon-y receptor
estrogen receptor
immunohistochemical method
coexistence
hypothalamus
rat
