摘要
目的探讨间充质干细胞(MSCS)移植对大鼠脊髓损伤后脑源性神经营养因子(BDNF)表达的影响。方法选取SD大鼠4只作骨髓间充质细胞的分离与培养,提取MSCS;制做脊髓横断损伤模型,细胞移植组24只,PBS(磷酸盐缓冲液)液组24只,空白对照组12只。于脊髓损伤后第7天,无菌条件下,细胞移植组以微量注射器缓慢注入含MSCS的培养液5μl,磷酸盐缓冲液组5μl,对照组未加任何干预因素。分别于术后7d、14d、28d麻醉下行心脏灌流固定取T10节段脊髓,细胞移植组与磷酸盐缓冲组取出损伤节段的脊髓,空白对照组于同一节段取出相应脊髓。应用免疫组化法观察MSCS移植后大鼠脊髓损伤区BDNF的表达变化。结果脑源性神经营养因子在正常大鼠脊髓组织中有一定表达,间充质干细胞移植术后7d、14d及28d,细胞移植组脑源性神经营养因子均高水平表达,与缓冲液组相比较差别明显,具有统计学意义。结论间充质干细胞在移植后通过上调脑源性神经营养因子的表达从而促进轴突的再生,可能是治疗脊髓损伤的重要机制。
Objective To observe the effects of mesenchymal stem cells（MSCS） transplantation on the brain-derived neurotrophic factors（BDNF） after the spinal cord injury（SCI） of rats,and to investigate the mechanism of repairing the SCI by MSCS transplantation. Methods Mesenchymal stem cell were cultured from the femur of adult SD rats and identified by immunohistochemistry. Seven days after the operation of SC1, the mesenchymal stem cells were transplanted into the injured spinal cord,sixty adult SD rats were randomly divided into three groups：（ group A）SCI cured with transplantation of mesenehymal stem cells; （group B）SCI received PBS solution, （group C） control group. Then the expression of brain-derived neurotrophic factors of the lesion and neighbour areas were examined through immunohistochemistry. Investigated the mechanism of repairing of the lesion and neighbor areas,and the mechanism of repairing the spinal cord after mesenehymal stem cells transplan- tation. Results Transplantation of mesenehymal stem cells enhanced the expression of brain-derived neurotrophic factors. There are significant difference among group A, B and C. Conclusion The expression of brain-derived neurotrophie factors in group A showed a higher level with a long time lasting than group B. It maybe one of the mechanisms of repairing the SCI by mesenehymal stem cells transplantation.
出处
《中国实用医药》
2008年第6期22-23,共2页
China Practical Medicine
关键词
脊髓损伤
骨髓间充质干细胞
细胞移植
脑源性神经营养因子
Spinal cord injury
Mesenchymal stem cells
Cell transplantation
Brain-derived neurotrophic factors
