摘要
背景:建立有效的完全性脊髓损伤动物模型是深入研究脊髓损伤的前提,只有建立标准的、可重复性高的实验动物模型才能择优选出治疗脊髓损伤的可行方案。目的:实验拟建立一种稳定的大鼠完全性脊髓损伤动物模型。设计、时间及地点:对照观察动物实验,于2007-11/2008-10在石河子大学药学院动物试验中心完成。材料:30只健康Wistar大鼠随机分成假手术组6只、实验组24只。方法:显露实验组大鼠T8～T12棘突及椎板,切除T9～10棘突及椎板,暴露相应脊髓段作为损伤区,采用大鼠脑定位仪自主设计改良Allen模型打击装置,予15g×20cm=2.94×10-2N重力打击大鼠T10节段脊髓,动物模型保证硬脊膜完整。假手术组仅同法暴露相应脊髓段,但不做打击。主要观察指标:造模后2,4,8周以斜板试验及BBB评分观察大鼠双后肢运动功能,以苏木精-伊红染色观察大鼠脊髓组织的变化。结果:假手术组大鼠苏醒后能站立行走,斜板试验角度均大于70°,BBB评分21分,脊髓结构正常。实验组大鼠造模后双下肢全瘫,2只大鼠表现为痉挛性瘫痪,5只大鼠表现出不同程度的自残现象。造模后2,4,8周斜板试验角度均小于30°,BBB评分均少于10分,随时间延长,部分大鼠可见后肢刺激性反射,但无主动性功能活动,局部脊髓结构破坏严重。结论:以2.94×10-2N重力打击大鼠脊髓可保证硬脊膜的完整,并获得稳定的完全性脊髓损伤动物模型。
BACKGROUND： Establishing an effective and complete animal model is a precondition for further research of spinal cord injury （SCI）, which can provide a basis for SCI treatment. OBJECTIVE： To establish a stable animal model of acute complete SCI in rats. DESIGN, TIME AND SETTING： A contrast control animal experiment was performed at the Center of Animal Experiment in College of Pharmaceutical, Shihezi University between November 2007 and October 2008. MATERIALS： Thirty healthy, Wistar rats were randomly divided into the control group （n=6）, and the experimental group （n=24）. METHODS： Rats in the experimental group were exposed processus spinosus and vertebral plate of T8 -T12, and cut processus spinosus, and vertebral plate of T9-10 of corresponding spinal cord were served as injured region. The weight drop device was designed with the cerebrum three-dimensional positioning indicator based on Allen’s model. All of experimental animals were attacked T10 segment （15 g×20 cm=2.94×10-2 N）. Be sure that the integrity and continuity of the dura in the experimental group were not damaged. There was no attack in the sham operation group. MAIN OUTCOME MEASURES： The motor function of both hind limbs were evaluated by oblique-plate test and BBB scores at 2, 4, and 8 weeks after operation. The pathological change of myeloid tissue was observed by hematoxylin-eosin staining. RESULTS： Rats in the sham operation group could walk after reviving, and the angle of oblique-plate test was more than 70°, with 21 scores. The micro-structure of spinal cord was normal. Rats in the experimental group were paralyzed completely after operation. Two rats showed spastic paralysis, and 5 rats showed different degree of self-mutilation. The angle of oblique-plate test was less than 30° with lower than 10 scores at weeks 2, 4, and 8 after operation. With the time increasing, some of rats had reflex activity to stimulation, but without active movement. The local inner-structure of spinal cord was damaged severe
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第33期6540-6543,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30760063)~~
