摘要
目的探讨硫酸软骨素酶ABC(chondroitinase ABC,ChABC)对大鼠脊髓损伤后轴突再生、髓鞘化和胶质瘢痕形成的影响。方法将72只雄性SD大鼠随机分为ChABC治疗组(A组)、生理盐水治疗组(B组)和假手术组(C组),每组24只。A、B组采用改良Allen撞击法制作大鼠T9中度脊髓损伤模型,分别在伤后1 h和之后连续7 d每天1次经蛛网膜下腔注射6μL浓度为1 U/mL ChABC和生理盐水;C组仅打开椎管,不损伤脊髓。术后1、7、14、28 d,采用BBB评分评定大鼠后肢运动功能恢复情况;取出损伤段脊髓组织,行HE染色和Nissl染色,并采用免疫组织化学染色检测髓鞘碱性蛋白(myelin basic protein,MBP)、生长相关蛋白43(growth associated protein 43,GAP-43)和胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的变化情况。结果术后各时间点C组BBB评分均明显高于A、B组(P<0.05);术后随时间延长A、B组BBB评分逐渐增加,14、28 d时A组BBB评分明显优于B组(P<0.05)。HE和Nissl染色显示术后各时间点A组脊髓组织形态和神经元数量均优于B组。术后各时间点A、B组MBP和GAP-43的积分吸光度(IA)值及GFAP染色阳性面积均高于C组(P<0.05),术后7、14、28 d时A组MBP和GAP-43的IA值明显高于B组(P<0.05),GFAP染色阳性面积明显小于B组(P<0.05)。结论 ChABC能有效改善大鼠脊髓损伤区域微环境,提高MBP和GAP-43表达并抑制GFAP表达,促进轴突再生与髓鞘化,抑制胶质瘢痕形成,促进神经功能恢复。
Objective To investigate the effects of chondroitinase ABC （ChABC） on axonal myelination and glial scar after spinal cord injury （SCI） in rats. Methods Seventy-two adult male Sprague Dawley rats were randomly assigned into ChABC treatment group （group A）, saline treatment group （group B）, and sham operation group （group C）, 24 rats in each group. In groups A and B, the SCI model was established with modified Allen＇s method and then the rats of groups A and B were administrated by subarachnoid injection of 6 laL ChABC （1 U/mL） and saline respectively at 1 hour after injury and every day for 1 week; the rats of group C served as control, which canal was opened without damage to spinal cord. At 1, 7, 14, and 28 days after operation, the locomotor functions were evaluated according to the Basso-Beattie-Bresnahan （BBB） score scale; and the spinal cord samples were harvested for HE staining, Nissl staining, and immunohistochemistry analysis to detect the change of myelin basic protein （MBP）, growth associated protein 43 （GAP-43）, and glial fibrillary acidic protein （GFAP） of the injured spinal cord. Results At different time points, the BBB score of group C was significantly higher than those of groups A and B （P 〈 0.05）, and the BBB score of group A was significantly better than that of group B at 14 and 28 days after operation （P 〈 0.05）. HE staining and Nissl staining showed that the morphous and the neuron number of the remainant injured spinal cord in group A were better than those in group B. The integral absorbance （IA） values of MBP and GAP-43 and the positive area of GFAP after SCI in groups A and B were significantly higher than those in group C at different time points （P 〈 0.05）, and the IA values of MBP and GAP-43 were significantly higher in group A than those in group B at 7, 14, and 28 days after operation （P 〈 0.05）, but the positive area of GFAP was significantly smaller in group A than that in group B （P 〈 0.05）. Conclusion T
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2013年第2期145-150,共6页
Chinese Journal of Reparative and Reconstructive Surgery
