摘要
用HPLC法对新疆药桑果实中白藜芦醇的含量进行了测定。色谱柱：Symmetry C18柱（4．6mm×150mm，5．0μm）；流动相：甲醇-水（50：50）；检测波长：306nm；柱温：室温；流速：1mL／min；进样量：20止。结果表明白藜芦醇在1～30mg／L时其峰面积与相应的质量浓度有良好的线性关系。回归方程为Y=48001x-47654，r=0．9997，加样回收率为95．31％，RSD=0．23％。该方法灵敏可靠，重现性好，初步测得药桑中白藜芦醇含量为7．875μg／g。
The HPLC method was used to determine the resveratrol content of drug mulberry of Xinjiang. The determination was carried out on C18 Colunm（4.6 mm×150 mm ,5.0μm ）with CH3OH-H2O（50： 50） as the mobile phase and detected at the 306 wave length. The resveratrol showed a good linear relationship at the range of 1-30 mg/L with r = 0. 9997, the average recovery of added sample was 95.31%, RSD = 0.23%. The method is simple, accurate , reproducible and the content of resveratrol is 7.875μg/g.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第B08期280-281,共2页
Natural Product Research and Development
