摘要
目的：建立高效液相色谱法测定苦味西葫芦果实中药西瓜苦苷的方法。分析其果实中药西瓜苦苷的含量。方法：采用Shim-Pack CLC-ODS（150mm×4.6mm，5μm）色谱柱，甲醇-水（52：48）为流动相，检测波长为238nm.柱温为35℃。结果：药西瓜苦苷在1.6～4.8/μg范围内线性关系良好.苦味西葫芦果实中药西瓜苦苷的含量为0.28％，RSD为1.27％。结论：该法稳定、简便，可用于苦味西葫芦药材的质量控制。
OBJECTIVE To establish an HPLC method for the determination of cucurbitacin E-2-O-β-D-glucopyranoside in the fructus of Cucurbita pepo. METHODS A Shim-Pack CLC-ODS column was used with methanol-water（52：48） as the mobile phase and the detection wavelength was at 238 nm. RESULTS The calibration curve was linear over the range from 1.6 to 4. 8μg. The content of Cucurbitacin E-2-O-β-D-glucopyranoside in the fructus of Cucurbita pepo was 0.28% and RSD was 1.27%. CONCLUSION The method is simple and stable. It can be used for the control of Cucurbita pepo.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第12期1694-1696,共3页
Chinese Journal of Hospital Pharmacy
