摘要
目的:建立沙棘茎中槲皮素、异鼠李素含量测定方法,并测定沙棘不同部位中两种苷元的含量。方法:以甲醇-0.4%磷酸(58:42)为流动相,370nm为检测波长,对甲醇提取酸水解后的样品进行含量测定。结果:沙棘茎、叶、果实中槲皮素的含量分别为:0.276、2.11、0.574mg/g,异鼠李素的含量为:0.463、1.60、0.729mg/g。结论:该方法简便、快速、结果可靠。
Objective：To establish the method of detecting the contents of quercetin and isorhamnetin,and to detectire the contents of the two types of aglycone in difforont parts of seabuckthorn.Methods ：The mobile phase consisted of Methanol-0.4% phosphoric aicd（58 ：42）.The detective wavelength was at 370nm.Detective the hydrlyed sample which was after methanol extract.Results ：The contents of quercetin and isorhamnetin in the stems,leares and fruit of seabuckthorn wore respectively：0.276,2.11,0.574,0.463,1.60,0.729mg/g.Conclusion ：This method is simple,quick and accurate.
出处
《辽宁中医药大学学报》
CAS
2010年第6期16-17,共2页
Journal of Liaoning University of Traditional Chinese Medicine
