摘要
对分离自近海沼泽地大米草根际的一株供试海洋细菌MWYL1进行了形态观察、生理特性检测以及16SrDNA序列分析。实验结果表明:该菌株属于海洋单胞菌属(Marinomonas),革兰氏染色呈阴性,直杆状,好氧生长,适于28℃生长。基于16SrDNA序列的Blast分析表明,菌株MWYL1与Marinomonas pontica和Marinomonas dokdonensis的序列相似性分别为97%和95%。通过基因组fosmid文库的构建,直接分离到一个产生黑色素的克隆,进一步亚克隆和测序后获得与黑色素产生相关的功能新基因(簇),并且对其进行了生物信息学的初步分析。
A marine bacterium MWYL1, originally isolated from the roots of Spartina anglica growing in a salt marsh near seaside, was identified as a member of the genus of Marinomonas via morphology characterization,physiological test and 16S rDNA sequencing and Blast analysis. The strain was short, rod, gram-negative, grew aerobically and optimally at 28℃. The analysis of 16S rDNA sequence suggests that the sequence similarity values are 97% and 95% with Marinomonas pontica and Marinomonas dokdonensis, respectively. One fosmid clone producing melanin was directly isolated by plating from the genomic library of Marinomonas MWYL1 .The novel functional gene cluster involved in melanin biosynthesis was screened after subcloning and sequencing of the 14kb insert in pUC18, further more, the putative functional genes was preminary analysed using bioinformaties.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第6期1118-1122,共5页
Microbiology China
基金
农学教学实验用微生物菌种资源标准化整理
整合及共享试点(2005DKA21208-6)
关键词
海洋细菌
黑色素
新基因簇
分离
Marine bacterium, Melanin, Novd gene cluster, Isolation
