摘要
文章报道了应用组织培养育苗技术对樟树家系进行快速繁殖的初步结果。试验表明:带腋芽的茎段外植体在添加细胞分裂素和生长素的MS培养基上可分化形成不定芽,但芽增殖和继代培养过程并不需要添加生长素。在DCR+BA3.0mg/L的培养基上增殖培养时,平均每个外植体增殖4.7个不定芽,高度小于1.5cm的不定芽的比例为74.1%;而在DCR+BA4.0mg/L的培养基上培养时,平均每个外植体增殖2.4个不定芽,高度大于1.5cm的枝芽比例可提高至58.1%。枝芽接种在DCR+IBA1.5mg/L的培养基上诱导生根培养28d的生根率为98%,移植的再生植株95%以上存活。
This paper deals with the preliminary results on tissue culture and rapid propagation of Cinnamomum camphora. The adventitious buds could be induced from shoot tips and axillary buds derived from family material MZMJ402 on MS medium supplemented with BA and NAA. Nevertheless, the trial results from subcultures indicated that the auxins had a disadvantageous effect on multiplication of adventitious buds. The optimum propagation medium selected from the experiments were DCR containing BA 3.0 mg/L or 4.0 mg,/L, in which the multiple coefficients reached 4.7 and 2.4 respectively. The trial identified that the optimum medium for root induction was DCR supplemented with IBA 1.5 mg/L and 98% of rooting percentage was obtained. The plantlet survival rate could exceed 95% provided that the nursery condition was acceptable.
出处
《广东林业科技》
2007年第5期35-39,共5页
Forestry Science and Technology of Guangdong Province
基金
广东省科技厅农业攻关项目2006B20201024的部分研究内容
关键词
樟树
组织培养
快速繁殖
Cinnamomum camphora （ L. ） Presl, tissue culture, rapid propagation
