摘要
象耳豆根结线虫(Meloidogyne enterolobii Yang and Eisenback)是海南岛独有的一种致病力很强的根结线虫。少孢节丛孢(Arthrobotrys oligospora Fresenius)HNQ11菌株是从海南岛香蕉(Musa sapientum Linn.)根围土壤分离到的1株捕食线虫真菌,本实验研究了其对象耳豆根结线虫的控制作用。结果表明,离体条件下HNQ11菌株能迅速产生捕食网和捕食环等捕食器官来捕食象耳豆根结线虫J2。其捕食率在J2加入HNQ11培养平板96h后即达83.5%;在盆钵控病实验中,每株番茄(Lycopersicon esculentum Mill.)幼苗用2g菌丝处理根部,根结形成比对照减少50%,根系鲜重增加147.3%。同时,在菌丝处理33d后还能在显微镜下观察到根片段上附着的HNQ11菌丝和分生孢子,重新分离菌丝和分生孢子还能在培养基上生长,说明HNQ11菌株在番茄根部具有定植能力。
The HNQ11 strain ofArthrobotrys oligospora, a nematode trapping fungus, was isolated from banana rhizosphere soil in Hainan Province and used for control of Meloidogyne enterolobii. Results showed that HNQ11 predated J2 individuals of M. enterolobii through development of trapping structures- trapping ring and trapping network. Its trapping efficacy was as high as 83.5 % after J2 individuals were poured onto the HNQ11 plates for 96 hrs. In in vivo experiments, treatment with 2 g mycelium per tomato plant decreased 50 % of gall formation and increased 147.3 % of the fresh weights of roots. Meanwhile, mycelium and conidia of HNQ11 on roots observed 33 days after treatment indicated that HNQ11 had colonial capacity on tomato roots.
出处
《热带作物学报》
CSCD
2007年第4期98-101,共4页
Chinese Journal of Tropical Crops
基金
中国热带农业科学院院基金资助[Rky0308]
中央级公益性科研院所基本科研业务专项资助项目(2007hzs1J011)
