摘要
目的观察不同剂量厄贝沙坦(Irb)对血管紧张素Ⅱ(AngⅡ)介导的肾小球系膜细胞(MCs)增殖及细胞周期的影响,探讨Irb抑制MCs增殖的可能机制。方法分离培养大鼠MCs,用10-6mol/LAngⅡ刺激MCs,将培养的大鼠MCs随机分为空白对照组、AngⅡ刺激组、高浓度Irb组(10-5mol/L)、中浓度Irb组(10-6mol/L)、低浓度Irb组(10-7mol/L)。采用四甲基偶氮唑盐(MTT)法及流式细胞术检测大鼠MCs增殖及细胞周期的变化。结果AngⅡ刺激组A值显著高于空白对照组,而高、中、低浓度Irb组A值均明显低于AngⅡ刺激组,且浓度越高作用越明显;AngⅡ刺激组较其他各组G0/G1降低,(S+G2)/M升高,而高、中、低浓度Irb组较AngⅡ刺激组G0/G1明显上升、(S+G2)/M明显降低,且浓度越高作用越明显;10-7～10-5mol/LIrb对MCs无明显细胞毒性作用。结论Irb可能是通过阻断AT1R介导的MCs的有丝分裂、增生及蛋白合成,从而抑制MCs的增殖。
Objective To investigate the effect of Irbesartan on the proliferation of mesangial cells （MCs）induced by angiotensin H in rats. Methods The MCs from healthy SD rats were cultured in vitro and divided into five groups： control group, Ang H group and 3 groups with various doses of Irbesartan. The proliferation of MCs was measured by MTT method and the cell cycle was observed by flow cytometry. Results Irbesartan inhibited Ang H -induced proliferation of rat MCs; Irbesartan also down-regulated （S＋G2）/M phase of MCs, Both effects showed a dose dependent relationship. Conclusion Irbesartan can inhibit proliferation of rat MCs, which is associated with decreased DNA synthesis.
出处
《浙江医学》
CAS
2007年第12期1262-1264,共3页
Zhejiang Medical Journal
基金
浙江省医药卫生科学基金(2005B005)
关键词
IRB
AngⅡ增殖
Irbesartan Angiotensin Ⅱ Proliferation
