摘要
目的探讨Cajal间质细胞（ICC）和肠胶质神经元数量在ANP豚鼠小肠组织中的改变及对肠动力的影响。方法24只豚鼠随机分为对照组、ANP24h组和48h组,各8只。采用腹腔注射20%L-精氨酸制作ANP模型。器官浸浴技术评价离体小肠的运动。HE染色观察胰腺和小肠组织病理改变。应用免疫荧光法检测ICC标志物酪氨酸激酶（C-KIT）和肠神经胶质细胞标志物的蛋白基因产物9.5（PGP9.5）。结果ANP24h和48h组胰腺组织呈明显水肿、坏死,小肠黏膜腺体结构紊乱,炎症细胞浸润。小肠运动的频率分别为（10.25±1.56）次/min和（10.37±1.41）次/min,运动振幅分别为（159.65±24.30）g＊s和（156.64±17.01）g＊s,较对照组明显下降;肌间丛ICC量从对照组的（0.273±0.03）%降至ANP组（0.104±0.04）%和（0.129±0.05）%,而肠神经元数量无明显变化。结论ANP豚鼠模型存在小肠动力功能的障碍,肠肌间丛Cajal间质细胞数量的减少可能是小肠动力功能抑制的重要因素。
Objective To investigate the change of distribution of Interstitial cells of Cajal（ICC） and the Intestine neuroglial cell in the gastrointestinal motility in a guinea pig model of acute necrotizing pancreatitis.Methods 24 Guinea pig were randomly assigned into Group A group B and group C.The guinea pig were subjected to acute necrotizing pancreatitis by intraperitoneal injection of 20% Larginine（2 g/kg） twice.Pancreas and small intestinal were resected at 24 h or 48 h.The spontaneous mechanical activity of the small intestinal segment was evaluated by organ bath technique.Immunopostivity of C-Kit and PGP9.5 at the level of the myenteric plexus was evaluated as markers of ICC and Intestine neuroglial cell,respectively.Results The pancreas after 24 h and 48 h showed obvious dropsy,necrosis,and the glandular organ of the tunica mucosa intestine showed disordered structure,inflammatory cell infiltration.The contractions frequency of the small intestine muscles showed a significantly reduction from（20.25 ± 3.51） /min to（10.25 ± 1.56）/min and（10.37 ± 1.41）/min（P 〈 0.01）,the amplitude of contractions decreased from（468.71 ± 60.3）gs to（159.65 ± 24.30）g＇s and（156.64 ± 17.01） gs.This change was associated with a significantly decrease（P 〈 0.01） of c-Kit immunopositivity from（0.273 ± 0.03）% to（0.104 ± 0.04）% and（0.129 ± 0.05）%.In contrast,the immunopositivity of PGP9.5 was not affected in any sever acute pancreasitis group.Conclusions Functional motility changes in ICC were present in the rat model of acute necrotizing pancreatitis,suggesting a central role of ICC in disrupting the normal gastrointestinal motility.
出处
《胰腺病学》
2007年第6期394-397,共4页
Chinese JOurnal of Pancreatology
关键词
胰腺炎
急性坏死性
小肠
间质细胞
神经元
Pancreatitis,acute necrotizing
Intestine,small
Stromal cells
Neurons
