摘要
目的:探讨针刺单穴及腧穴配伍对糖尿病胃轻瘫(Diabetic gastroparesis,DGP)大鼠胃窦Cajal间质细胞(Interstitial Cells of Cajal,ICC)的影响。方法:选取雄性SD大鼠50只,随机分为正常组、模型组、多潘立酮组、足三里组、中脘+足三里组,每组10只。以链脲佐菌素(STZ)腹腔注射诱导DGP大鼠模型。采用免疫组化技术观察各组大鼠ICC含量的改变。结果:与正常组相比,模型组大鼠胃窦组织ICC含量明显减少(P<0.01);与模型组比较,多潘立酮组(P<0.01)、足三里组(P<0.05)、中脘+足三里组(P<0.01)大鼠胃窦ICC含量明显提高,且中脘+足三里组优于足三里组(P<0.05)。结论:针刺促进DGP大鼠胃窦ICC含量恢复,腧穴配伍组优于单穴组。
Objective： To explore the effects of acupuncture at single acupoint and combined acupoints on antral interstitial cells of Cajal（ ICC） in diabetic gastroparesis model rats. Methods： 50 SD male rats were randomly divided into 5 groups with 10 rats in each,namely normal group,diabetic gastroparesis model rats group（ the model group）,domperidone group,acupuncture at ST36 group and acupuncture at ST36 ＋ CV12 group. The rats were intraperitoneally injected with steptozotocin to set diabetic gastroparesis model. Treatments for each group lasted for 5 weeks. Numbers of ICC were detected by immunohistochemical methods. Results： The number of ICC in the model group were significantly reduced（ P 〈 0. 01）,compared with normal group,while numbers of ICC in domperidone group（ P 〈 0. 01）,acupuncture at ST36 group（ P 〈 0. 05） and ST36 ＋ CV12 group（ P 〈 0. 01） were improved significantly,compared with the model group. Numbers of ICC in the acupuncture at ST36 ＋ CV12 group was increased remarkably superior to the acupuncture at ST36 group（ P 〈 0. 05）. Conclusion： Acupuncture could improve the numbers of antral interstitial cells of Cajal in diabetic gastroparesis model rats,and acupuncture at combined acupoints was better than that of the single acupoint.
出处
《世界中医药》
CAS
2016年第2期214-218,共5页
World Chinese Medicine
基金
国家重点基础研究发展计划("973"计划)项目(编号:2014CB543100)
吉林省卫生厅项目(编号:2014Q048)
关键词
糖尿病胃轻瘫
针刺
腧穴配伍
CAJAL间质细胞
Diabetic gastroparesis
Acupuncture
Acupoint combination
Interstitial cells of Cajal
