摘要
利用红酵母静息细胞转化3,5-双三氟甲基苯乙酮,并对影响红酵母细胞转化率的因素进行考察。采用静息转化法,考察不同有机溶剂、有机溶剂体积比、细胞通透剂、pH值、转化时间等因素对转化率的影响。结果表明,以辛烷作为有机溶剂,体积分数为20%,pH7.0,不使用细胞通透剂,葡萄糖作为辅助底物,转化时间为36h时,转化效率最高,可达81.7%。
To biotransform 3, 5-Bis （trifluoromethyl） acetophenone using resting cell of rhodotorula and investigate all the factors involved in the transformation, such as the kind of organic solvent, the best ratio of aqueous/ organic , permeabilization, pH and transforming time. The results showed that the maximal transforming ratio was obtained in 36 h under the optimum conditions as follows： the organic solvents 1-octane, the ratio of organic solvent 20%, co-substrates glucose, pH7. 0.
出处
《药物生物技术》
CAS
CSCD
2007年第6期411-414,共4页
Pharmaceutical Biotechnology
