摘要
以酵母细胞不对称还原苯乙酮合成S-α-苯乙醇为模型反应,研究了酵母细胞催化芳香酮的不对称还原情况.首先考察了底物和产物对细胞的毒性情况及对反应的不利影响;再在此基础上研究通过引入树脂吸附控制底物和产物浓度以避免较高浓度底物和产物对细胞的毒性,进而提高反应时-空产率.实验发现苯乙酮和α-苯乙醇对酵母细胞活性具有显著的毒害,抑制反应的活性;引入树脂吸附可显著促进反应的进行,使用杭州争光树脂厂的ZG3#树脂可获得最好的效果;当使用适量的树脂时底物初始浓度即使提高到70mmoL·L-1还可以获得较好的产物收率,这比未引入树脂吸附时的底物浓度提高了近1倍.
Optically active aryl alcohols are one kind of the most important chiral building blocks for many single enantiomer pharmaceuticals. Asymmetric reduction of corresponding prochiral ketone to chiral alcohol by active yeast cells is one of the most promising routes, but the space-time productivity is very low due to the toxicity of the substrate and product to the cell, which prohibits the reaction from proceeding at a high substrate concentration. The toxicity of aryl ketone to yeast cell and increasing the initial substrate concentration by introducing macropore adsorbing resin to avoid the toxicity to the cell was studied, when asymmetric reduction of acetophenone （ACP） to chiral a-phenylethyl alcohol （PEA） by Saccharomyces cerevisiae was chosen as the model reaction. The experiments showed that the substrate and product did have obvious toxicity to yeast cell. This reaction could be remarkably improved with introducing the appropriate kind of resin. The initial ACP concentration could be increased to 70 mmol · L^-1 when the appropriate amount of resin was used.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第10期2388-2392,共5页
CIESC Journal
基金
国家自然科学基金项目(20276065)
武汉科技大学基金项目(2005XY15).~~
关键词
不对称还原
手性醇
S-α-苯乙醇
树脂吸附
酵母细胞
asymmetric reduction
chiral alcohol
S-a-phenylethyl alcohol
resin adsorptionSaccharomyces cerevisiae
