摘要
蔗糖浓度对聊红槐试管苗的增殖倍数的影响较小,但影响试管苗的生长形态,在WPM培养基中蔗糖浓度由原来20 g.L^-1增加到60 g.L^-1时,叶片由皱褶变为正常,颜色变为深绿。经过以下培养程序可以建立叶片再生体：将聊红槐增殖分化苗接种于WPM（附加6-BA 2.0 mg.L^-1＋NAA 0.05 mg.L^-1＋蔗糖20-60 g.L^-1＋琼脂7.0 g.L^-1）上,继代培养20 d,获得分化型试管苗;剪取叶片投入1 mg.L^-1Vc无菌水浸泡1-3 m in,接入WPM（附加6-BA 2.0 mg.L^-1＋NAA 0.1 mg.L^-1＋蔗糖40 g.L^-1＋琼脂7.0 g.L^-1）上,光照培养30-40 d,获得剪切口组织脱分化;环境控制（光照时间12 h,暗培养12 h,光强2000 1x,温度25℃;黑暗温度15℃,pH值5.8）下培养20-30 d,诱导出绿色愈伤组织;转接WPM培养基（附加6-BA 4.0 mg.L^-1＋NAA 0.05 mg.L^-1＋GA 2 mg.L^-1＋蔗糖60 g.L^-1＋琼脂7.0 g.L^-1）上,培养后由绿色愈伤组织萌发出不定芽,再生不定芽率为89%。
The propagational multiple of Sophora japonica f. ‘Liao Hong＇ tube seedlings was less affected by the concentration of sucrose, but the concentration of sucrose affected the growth morphology of tube seedlings. When the sucrose concentration increased from 20 g · L^-1· to 60 g · L^-1 in WPM media, the leaves turned to normal from crinkled and became dark green. The procedures of leave regeneration culture are as follows： The differentiated seedlings of S. japonica were inoculated on WPM （ with 6 - BA 2.0 mg· L^- 1 ＋ NAA 0. 05 mg · L^- 1 ＋ sucrose 20 - 60 g · L^-1 ＋ agar 7.0 g ·L ^-1 ）, after cultured for 20 days, the differentiated tube seedlings were obtained. The leaves sheared were put into asepsis water with 1 mg · L^-1 Vitamin C and soaked for 1 - 3 minutes, then planted on toWPM （with6-BA2.0mg. L^-1＋NAA0. 1 mg· L^-1＋sucrose40g· L^-1＋agar7.0g· L^-1）, and cultured under illumination for 30- 40 days, the differentiated cutting tissues were got. Cultured under controlled environment （illumination time 12 hours, dark culture for 12 hours, light intensity 2 000 Ix, temperature 25℃, dark culture temperature 15℃, pH 5.8） for 20 - 30 days, the green callus were induced; then they were transplanted onWPMmedia （with6-BA4.0 mg. L^-1 ＋NAA0. 05 mg. L^-1 ＋GA2 mg. L^-1 ＋sucrose60 g. L^-1 ＋agar 7.0 g · L^-1）, the adventitious buds were germinated from green callus tissues, and the regenerated adventitious buds rate was 89%.
出处
《林业科学研究》
CSCD
北大核心
2007年第6期854-858,共5页
Forest Research
关键词
影响因素
聊红槐
叶片
再生体
affecting factors
Sophora japonica f. ＇ Liao Hong＇
leave
regeneration
