摘要
系统研究了槐树叶片、子叶及花药的培养方法和植株再生，获得了大量的试管苗，建立了槐树二倍体和单倍体植株快速繁殖的培养程序。实验表明在MS附加高浓度比值BA／IBA或2，4-D的培养基中可诱导各种外植体在短期内产生大量的不定芽和试管苗，但同时，又诱导产生了许多超度含水态苗。在培养过程中，BA浓度先高后低，既可获得大量的试管苗，又相对降低了超度含水态苗的比率，是槐树快速繁殖程序中的重要一环。组织学观察显示，槐树子叶切块培养中形成的胚状体来自于子叶的表皮及叶肉细胞，通过单细胞起源和多细胞出芽两种方式产生。
The culture methods and plantlet regeneration from leaf,cotyledon explants and theanther culture of Sophora japonica L. were systematically studied and a large number ofplantlets were obtained. Some procedures of rapid propagation for diploid and haploidplantlet production were established. On MS medium supplemented with a higherconcentration ratio of BA/IBA or 2, 4--D could induce the explants to produce a lot ofadventitious buds in a short time,but meantime many hyperhydric shoots fromed on this kindof medium. One of the keys in the rapid propagation procedure of Sophora japonica L.was using higher concentration of BA in the early period of culture and then reduced oreliminated it. Histological observation of embryogenesis in the cotyledon explant cultureshowed that the embryoids originated from the epiderm and mesophyll cells throughboth single--cell initiation and multicellular budding ways.
出处
《西北植物学报》
CAS
CSCD
1997年第5期1-6,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省自然科学基金
关键词
槐树
组织培养
植株再生
胚胎发生
Sophora japonica L.,tissue culture,plantlet regeneration,embryogenesis
