摘要
目的:检测突出的腰椎间盘髓核中FasL受体的表达情况,探讨其与腰椎间盘突出症发生与发展的关系。方法:根据美国矫形外科学会(AAOS)及国际腰椎研究会(ISSLS)分类,将20例术中取出的腰椎间盘突出症患者髓核标本分成退变组(10例)、脱出组(10例),并以7例外伤性脑死亡青年患者作为正常对照组,应用免疫组化法检测髓核中FasL受体表达情况并通过电镜查找凋亡细胞。结果:(1)FasL受体主要定位于细胞膜,呈均匀的棕黄色细颗粒状或粗细不等;(2)3组FasL阳性表达率明显不同(P<0.05);(3)Fas阳性表达率的高低同年龄有关(r=0.4102,P<0.05),但与性别及病程无关;(4)电镜可观测到细胞凋亡的一些形态学变化。结论:腰椎间盘、突出髓核细胞中存在FasL受体的表达,突出类型不同,FasL受体表达的程度也不同,其异常表达诱导了髓核细胞的凋亡,可能在腰椎间盘突出症的发病过程中起着重要作用。
Objective： To investigate the cell type that expressed gene proteins FasL and the evidence of apoptosis of the disc cells in herniated lumbar disc tissues. Methods： Twenty herniated lumbar disc tissues （contained disc n=10 and noncontained disc n=10）and seven normal disc tissues were examined to investigate the cell type that expressed FasL and any evidence of apoptosis of the disc cells by using immunohistochemical S-P method and under electron microscope respectively. The percentage of FasL-positive disc cells was calculated and compared with clinical and radiologic data.Results：（ 1 ）FasL was expressed in the cell membrane of the disc cells. （2）There was a significant difference in the percentages of FasL-positive disc cells among the contained, noncontained and normal discs（P〈0.05）. （3）The percentages of FasL-positive disc cells correlated significantly with the patient＇s ages（r=0.4102,P〈0.05）, but not with the sex,duration of sciatica（P〉0.05）. There was some evidence of apoptosis of the disc cells under electron microscope.Conclusion： The expression of FasL on disc cells in herniated disc tissue is identified. The current results indicate that the abnormal expression of FasL on disc cells may lead to FasLmediated apoptosis. The degrees of expression of FasL are varied depending on the types of herniation.
出处
《南通大学学报（医学版）》
2007年第5期337-339,共3页
Journal of Nantong University(Medical sciences)
关键词
腰椎间盘突出症
髓核细胞
凋亡
FASL
免疫组织化学
Protrusion of intervertebral disc
Apoptosis disc cell
FasL receptor
Herniated lumbar disc
Immunohistochemistry
