摘要
本文旨在研究5种等氮等能饲料中,维生素A添加水平对凡纳滨对虾(Penaeus vannameiBoone)生长、饲料利用、体组成成分及非特异性免疫反应的影响。试验选用平均初始体重为(0.069±0.005)g的凡纳滨对虾450尾,随机分为5个处理组,每组90尾,每组设3个重复,每个重复30尾。试验采用单因素设计,以维生素A醋酸酯为维生素A源,用添加不同水平维生素A(添加量分别为0、18、365、4和72 mg/kg)的饲料喂养凡纳滨对虾,试验期11周。结果表明:在养殖前4周,饲料中添加维生素A显著促进凡纳滨对虾的生长(P<0.05),提高其对饲料利用效率(P<0.05),而对对虾的成活率不产生显著的影响(P>0.05);第11周的结果表明,饲料中添加维生素A并不能显著影响凡纳滨对虾的生长、饲料利用效率和成活率(P>0.05)。维生素A对对虾体脂肪、蛋白质含量的影响显著(P<0.05),对对虾体水分和灰分含量无显著影响(P>0.05)。维生素A对对虾血清中溶菌酶活力、酚氧化酶活力和血液红细胞数量的影响显著(P<0.05),饲料中未添加维生素A或过量添加(超过36 mg/kg)均导致血清中溶菌酶活力、酚氧化酶活力和血细胞总数显著下降(P<0.0 5),而对血清超氧化物歧化酶活力无显著影响(P>0.0 5)。用折线回归模型分析饲料维生素A添加量与对虾增重率的变化关系,表明在试验前4周,凡纳滨对虾饲料中维生素A的适宜添加量为2 2.5 0 mg/kg;1 1周试验期间,凡纳滨对虾饲料中维生素A适宜添加量为1 8 mg/kg;以溶菌酶、酚氧化酶活力为指标,凡纳滨对虾饲料中维生素A最适添加量为5 9.5 1 mg/kg。
To study the effects of different dietary levels of vitamin A on growth performance, feed utilization, body composition and non-specific immunological responses of juvenile shrimp （Penaeus vannamei Boone）, an experiment was conducted using a total of 450 juvenile shrimps to investigate the effects of adding vitamin A （0, 18, 36, 54 and 72 mg/kg respectively） on growth, feed utilization, body composition and non-specific immunological responses of juvenile shrimp （Penaeus vannamei Boone）. Juvenile shrimps were randomly divided into 5 treatments with 3 replicates each （90 for each treatment, 30 for each replicate）. Five iso-nitrogenous and iso-energies diets were formulated as experimental diets, and the experiment lasted for 11 weeks. The results showed： In 4-week feeding trial, the diet with vitamin A supplementation could significantly increase the growth rate, feed convention ratio （FCR） and protein efficiency ratio （PER） of juvenile shrimp （ P 〈 0.05）, While the survival rate was not affected （P〉0.05）. Up to 11-week feeding trial, there was no significant effect of increasing dietary vitamin A on growth, the survival rate, FCR and PER of shrimp （P〉0.05）. The whole body lipid and protein content were significantly affected by the dietary vitamin A supplementation levels （P〈0.05）, while there was no significant effect of vitamin A on body moisture and ash content. The activity of superoxide dismutase （SOD） of shrimp serum was not affected by the dietary vitamin A levels （P〉0.05）. The phenoloxidase activity, lysozyme activity and red blood count decreased significantly when the dietary without vitamin A supplementation or more than 36 mg/kg were supplemented （P〈0.05）. Based on broken-line regression analysis of weight gain rate at 4 wk and 11 wk , lysozyme activity and phenoloxidase activity（at 11wk）, the optimal dietary supplementation levels of vitamin A for this diet formulation was 22.5 mg/kg, 18.0 mg/kg and 59.51 mg/kg, respectively. [Chin
出处
《动物营养学报》
CAS
CSCD
北大核心
2007年第6期698-705,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广东省科技攻关项目(2002C20325)
关键词
凡纳滨对虾
维生素A
生长
饲料利用
体组成
非特异性免疫
Penaeus vannamei Boone
Vitamin A
Growth
Feed utilization
Body composition
Non-specific immunological responses
