摘要
目的:观察帕金森病(PD)大鼠中脑黑质外液及红细胞内Mg^(2+)浓度的改变。方法:采用6-羟基多巴(6-OHDA)损毁制备偏侧PD大鼠模型24只,分4组各6只,分别于造模后第7、14、21、28天采集大鼠动脉血中的红细胞,采用微透析技术收集中脑黑质细胞外液,火焰原子吸收光谱法测定红细胞内和黑质细胞外液Mg^(2+)含量。选5只健康大鼠作为对照组,进行相同测定。结果:各时点PD组红细胞内Mg^(2+)含量均显著低于对照组(P<0.05),且随病程延长而逐渐下降(P<0.05);但PD组中脑黑质细胞外液Mg^(2+)含量仅在第21、28天明显低于对照组(P<0.05)。结论:Mg^(2+)缺乏与PD的发生及病程进展有一定关联。
Objective： To explored changes in magnesium levels in red blood cells and extracelluar fluid of substantia nigra in a rat model of Parkinson＇s disease. Methods： Twenty-four rats were stereotactically injected with 6OHDA to establish the unilateral model of Parkinson＇s disease. Blood samples and extracellular fluid of the substantia nigra were extracted from six PD rats on the 7th, 14th,21st and 28th days after the injection. Contents of magnesium were detected with atom-absorption spectrophotometer technique. Samples of 5 normal rats were subjected to the same assay as controls. Results： When compared to the control group, the magnesium concentrations in RBC decreased significantly in a time-dependent manner （P〈0. 05）, whereas those in the extracellular fluid of the substantia nigra have decreased statistically only on the 21st, 28th days in PD rats （P〈0.05）. Conclusion： Deficiency of magnesium correlates with the pathogenesis of Parkinson＇s diseases to some extent.
出处
《神经损伤与功能重建》
2007年第6期333-335,共3页
Neural Injury and Functional Reconstruction
