摘要
目的 在已制备的微流控芯片系统上建立基于顺磁微球的免疫检测方法，并将此方法用于检测促甲状腺激素（TSH）和甲状腺素（T4）。方法 采用数字激光刻蚀技术制备微流控芯片，以luminol-H2O2为发光体系，用双抗夹心法测定TSH，用免疫竞争法测定T4。结果 该检测系统可以在25min内完成检测，所需的标本和抗体试剂量为10山，TSH检测范围为0～50μIU／ml，批内CV平均为4．9％，批间CV平均为4．9％，平均回收率为93．74％；T4检测范围为9．375～300ng／ml，批内CV平均为4．9％，批间CV平均为4．7％，平均回收率为91．27％。结论 本方法检测TSH和T4具有操作简便、节约时间、节省试剂、稳定性好、准确度高的优点。
Objective To establish an immunoassay based on microfluidic chip for detection of TSH and total T4. Methods The direct-write laser micromachining technology was used to fabricate microfluidic chip, luminol-H2O2 as luminator. This method and Roche E2010 electrochemiluminescence immunoassay were used to examine TSH by double-antibody sandwich method and total T4by competitive principle in 50 serum samples collected at our outpatient clinic. Results The detection was completed within 25 min. Ten microliter sample and reagent was required. The detection range of TSH was 0 - 50 la, IU/ml and its average intraassay and interassay precision was 4.7% and 4.6% respectively and its average recovery rate was 95.3%. The detection range of T4 was 9.375 -300 ng/ml, and its average intraassay and interassay precision was 4.9% and 4.7% respectively and its average recovery rate was 91.27%. Conclusion Rapid detection of TSH and T4 by microfluidic chip-based immunoassays is convenient, stable and accurate and of less reagent and time consuming.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第23期2254-2256,共3页
Journal of Third Military Medical University
关键词
微流控芯片
免疫顺磁微球
化学发光
microfluidic chip
immunoassay magnetic microbead
chemiluminescence
