摘要
目的：建立一种可用于免疫分析的微流控芯片系统。方法：采用激光直接加工法制备微流控芯片，建立基于超顺磁珠的化学发光微流控免疫分析系统，用于甲胎蛋白（AFP）的测定。结果：芯片系统可在20min内完成AFP的分析，所需要的标本量和试剂量为5μl，线性范围为1～800ng／ml，批内变异系数（CV）平均为6，3％，批间CV平均为7．8％，回收率为94．3％。结论：微流控芯片系统是一种快速、耗费小、可重复使用的AFP测定方法。
Objective：To establish a microfluidic chip system for immunoassay. Methods：We use diect-writing laser micromachining technology to fabricate microfluidic chip, establish microfluidic chemi- luminescence immunoassay system based on superparamagnetic microbeads, and use this system to detect alpha-fetoprotein. Results：We can complete the analysis within 20 minutes with 5 μl sample or reagent, the linear range was 1 -800 ng/ml with average intra-assay and inter-assay precision at 6.3% and 7.8% , respectively and average recovery at 94.3%. Conclusion ： The microfluidic chip system is a time-saving, cost-low and convenient method for AFP analysis.
出处
《医学研究生学报》
CAS
2006年第11期967-969,共3页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30270407)
关键词
超顺磁珠
化学发光
微流控芯片
免疫分析
Superparamagnetic microbeads
Chemiluminescent
Microfluidic chip
Immunoassay
