摘要
目的通过检测人类白细胞抗原(HLA)Ⅱ类等位基因DRB1*0401～*0408,分析基因频率与临床表现的关系,初步探讨HLA-DRB1多态性与自身免疫性肝炎和原发性胆汁性肝硬化的相关性。方法按自身免疫性肝炎和原发性胆汁性肝硬化的诊断标准筛选病例。以31例江苏地区汉族人自身免疫性肝病患者(自身免疫性肝炎12例,原发性胆汁性肝硬化19例)为研究对象,以42例健康人为对照组。采集静脉血,用酚-氯仿法提取全血DNA。用序列特异性引物聚合酶链反应(PCR- SSP)技术检测HLA-DRB1*0401～*0408等位基因频率。结果自身免疫性肝病患者HLA-DRB1*0404等位基因的频率(35.5%)明显高于健康人(7.1%),RR为7.15,(P<0.005),其他各等位基因频率在2组间虽有不同,但无统计学意义的显著性差异。自身免疫性肝炎组和原发性胆汁性肝硬化组之间HLA-DRB1*0404及其他各等位基因频率无统计学意义的显著性差异。结论HLA-DRB1*0404等位基因可能与自身免疫性肝病的发生有关联。
Objective The aim of this study is to explore the association between human leukocyte antigen （HLA） alleles type Ⅱ and the pathogenesis of autoimmune liver diseases （ALD）. Methods The frequencies of HLA-DRB1 ＊ 0401- ＊ 0408 in research group （31 patients with ALD） and control group（42 healthy subjects） were detected by PCR-SSP （polymerase chain reaction-sequence-specific primer）. Both the patients and the healthy came from Han nationality in Jiangsu area. Results The frequency of HLA-DRB1 ＊ 0404 in ALD patients was markedly higher than that of the controls （35; 5% vs 7.1%, RR 7.15, P〈0. 005）. The frequencies of other alleles exhibited slightly shifted but no significant statistical difference was found. Conclusion HLA-DRB1 ＊ 0404 may be associated with ALD and may be one of the susceptible genes for ALD.
出处
《实用临床医药杂志》
CAS
2007年第5期44-48,共5页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金资助项目(60477031)
江苏省社会发展基金资助项目(BS2005012)
