摘要
目的研究硝苯啶（Ｎｉｆ）、脑益嗪（Ｃｉｎ）、Ａ２３１８７和氯丙嗪（ＣＰＺ）对３Ｔ６成纤维细胞增殖的影响．方法以含１０％小牛血清的ＲＰＭＩ１６４０培养基培育３Ｔ６细胞，分别加入３种浓度的Ｃｉｎ，Ｎｉｆ，Ａ２３１８７和ＣＰＺ，处理２４ｈ后用图像分析仪和流式细胞仪检测３Ｔ６细胞的ＤＮＡ，ＲＮＡ和总蛋白以及细胞周期．结果Ｎｉｆ剂量依赖性地延迟３Ｔ６细胞Ｇ２期向Ｍ期的进展；Ｃｉｎ，Ａ２３１８７和ＣＰＺ分别呈剂量依赖性地抑制３Ｔ６细胞Ｇ１期向Ｓ期的进程；Ｎｉｆ，Ａ２３１８７和ＣＰＺ促进细胞的ＲＮＡ合成，Ｃｉｎ抑制细胞ＤＮＡ及ＲＮＡ的合成；Ａ２３１８７和ＣＰＺ（１０μｇ）抑制细胞蛋白质的合成，Ｃｉｎ（３０μｇ）及ＣＰＺ（５μｇ）促进细胞蛋白质合成．结论Ｎｉｆ等通过对细胞周期进程的延迟及引起细胞不平衡生长，抑制３Ｔ６成纤维细胞的增殖．
AIM To study the effects of Cin, Nif, A 23187 and CPZ on the proliferation of 3T6 fibroblasts. METHODS 3T6 cells were cultured in RPMI 1640 medium supplemented with 10% FCS. Three different concentrations of Cin, Nif, A 23187 and CPZ were added separately to each test sample, and 24 hours later 3T6 cells were measured by ICM and FCM to determine the DNA, RNA, total protein content and cell cycle. RESULTS Nif could delay the progression of 3T6 cells from G 2 to M phase in a dose dependent manner. Nif, A23187 and CPZ accelerated the synthesis of RNA and Cin inhibited the synthesis of RNA and DNA. A 23187 and CPZ (10μg) inhibited the synthesis of protein, but Cin (30μg) and CPZ (5μg) promoted the synthesis of protein. CONCLUSION Calcium antagonists inhibit the proliferation of 3T6 cells by delaying the progression of cell cycle and inducing unbalanced cell growth.
