摘要
目的：探讨川芎嗪对肝星状细胞（hepatic stellate cells,HSC）表达α-平滑肌肌动蛋白（α-smooth muscle actin,α-SMA）、核因子-κB（NFκ-B）的影响。方法：体外培养人肝星状细胞,免疫细胞化学法检测HSC中α-SMA的表达以及NFκ-B表达的核迁移。结果：在50-800mg/L浓度范围内,川芎嗪处理组α-SMA表达量不同程度减少,且抑制作用随剂量增加而增强;川芎嗪处理组NF-κB表达量较少并且主要位于细胞质,很少发生核迁移。结论：川芎嗪可以使活化的HSC中α-SMA、NFκ-B表达下降。
Objective： To observe the effects of tetramethylpyrazine（TMP） on expression of α-smooth muscle actin（α-SMA） and nuclear factor-κB （NF-κB） in human hepatic stellate cells（ HSCs）, to investigate the effect of TMP against hepatic fibrosis. Methods：HSCs cultured in vitro were divided into negative control group, positive control group（ cultured with matrine 500mg/L） , experimental group（ cultured with different concentrations of TMP 50mg/L,100mg/L,200mg/L,400mg/L,800mg/L）. Immunocytochemical S-P method was used to detect the expression of α-SMA and proteins of NF-κB p65. Results： In the range of 50 - 800mg/L, TMP had significant inhibitive effects on the expression of α-SMA in a dose-dependent manner compared with the negative control group and positive control group. The positive expression of NF-κB in negative control group was in the plasma and nuclear of HSCs. In the positive control group and experimental groups, the expression of NF- κB was decreased and little nuclear transfer was observed. Conclusion： The positive expression of α-SMA and NF-κB in HSCs was down- graded by TMP. And the activation of HSCs was inhibited. TMP has depressant effect on hepatic fibrosis.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第5期76-78,共3页
Pharmacology and Clinics of Chinese Materia Medica
