摘要
利用紫外线对氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)进行诱变处理,获得了对SO32-和Fe2+具有较高转化效率的突变菌株。结果表明,突变菌株将Fe2+完全氧化所需时间从诱变前的48 h缩短为24 h,并且具有较宽酸碱度适应范围。在突变菌株与Fe2+共存时,SO32-的氧化速率明显高于化学氧化,且以间接氧化为主,细菌对Fe2+的氧化是反应过程的限制步骤。当SO32-的初始浓度为2.7 g.L-1、Fe2+的浓度为0.43 g.L-1时,SO32-的转化速率为0.0153 g.L-1.min-1。
Acidithiobacillus ferrooxidans was treated by UV to obtain a mutant strain which has a high conversion efficiency of SO3^2- and Fe^2+. The results showed that the mutant strain reduces the time for the perfect conversion of Fe^2+ from 48 hours to 24 hours. The biological oxidation rate of SO3^2- was obviously higher than the chemical oxidation rate when Fe^2+ coexis.ed with the mutant strain. The bacteria did not directly oxidate SO3^2- , and the oxidization of Fe^2+ by the bacteria seemed to be the key step of oxidation of SO3^2-. The conversion rate of SO3^2- was 0. 0153 g · L^-1 · min^-1 when the initial concentration of SO3^2- and Fe^2+ was 2.7 g · L^-1 and 0. 43 g · L^-1, respeetively.
出处
《四川大学学报(工程科学版)》
EI
CAS
CSCD
北大核心
2007年第2期82-86,共5页
Journal of Sichuan University (Engineering Science Edition)
基金
国家自然科学基金资助项目(50278059)