摘要
目的:观察不同时程电针(EA)镇痛的累积效应,及其与血浆促肾上腺皮质激素(ACTH)、β-内啡肽(EP)及皮质醇(COR)含量变化的关系。方法:Wistar雌鼠110只,随机分为正常对照组(n=10)、CCI组(n=10)、CCI+EA组(n=30)、去卵巢(OVX)+CCI组(n=30)、OVX+CCI+EA组(n=30),后3组又各分为EA 2次(2 t)、2周(2 w)和3周(3 w)亚组(时程),各10例。上述后3组动物去除双侧卵巢45 d后进行水迷宫测试,检查动物学习记忆能力。结扎坐骨神经造成慢性疼痛模型。电针双侧"足三里"-"阳陵泉"穴(2/15 Hz,1 mA,30 min),1次/d,不同组分别电针2 t、2 w、3 w。用辐射热刺激测定大鼠缩腿潜伏期(PWL)作为痛阈,用健侧和患侧PWL的差值做组间比较。深度麻醉断头采血,用放射免疫法测定ACTH、β-EP及COR的含量。结果:与正常对照组比,CCI各组PWL差值均明显较大(P<0.05);与CCI组比较,CCI+EA组3 w时段差异有显著性意义(P<0.05),接近正常对照组。与OVX+CCI组比较,OVX+CCI+EA组2 w、3 w的PWL差值均明显减小(P<0.05),疼痛减轻。CCI+EA和OVX+CCI+EA两组比较,前者多数时程的PWL差值显著低于后者(P<0.05),说明没有去卵巢大鼠电针的镇痛效果明显较优。在单纯CCI模型上,CCI后,血浆-βEP、ACTH及COR含量均没有明显改变;给予电针后,-βEP、ACTH的水平没有明显改变,COR含量明显增加(P<0.05)。在OVX+CCI复合动物模型上,血浆-βEP的浓度2 w和3 w时均明显高于正常对照组(P<0.05),ACTH含量没有明显变化,COR含量减低或明显增加(P<0.05);与OVX+CCI组比,OVX+CCI+EA组电针2 t2、w组其-βEP浓度明显下降(P<0.05),3 w也有所减少,ACTH的水平电针2 t和3 w明显增加(P<0.05),2 w组明显下降(P<0.05),COR的浓度2 w明显降低(P<0.05)。说明在单纯CCI模型上,当出现明显针刺镇痛效应时,仅血浆COR的浓度增加;在OVX+CCI复合动物模型上,当出现明显针刺镇痛累积效应时,血浆-βEP的水平下降或明显下降,COR的浓度降低,血浆ACTH含量的增、减�
Objective： To observe the regularity of electroacupuncture （EA） analgesia and to analyze its relation to hypothalamus-pituitary-adrenal cortex axis function in chronic constrictive injury （COl） and ovariectomy （OVX） rats. Methods： A total of 110 Wistar rats anesthetized with mixture solution of 1.5% chloralose （50 mg/kg） and 25% urethrane （420 mg/kg, i. p. ） were randomized into control （n=10）, CCl （n =10）, CCl＋EA （n=30）, OVX＋CCl （n=30）,OVX＋CCl＋EA （n=30） groups, and the later 3 groups were further divided into 2 times （2 t）, 2 weeks （2 w）and 3 w time courses, with 10 cases in each. Forty-five days after OVX, Morris water maze test was conducted to evaluate the OVX rats＇ memory-learning ability. Chronic pain model was established by ligating the right sciatic nerve with a piece of suture. EA （2/15 Hz, 1 mA） was applied to bilateral ＂Zusanli＂ （ST 36）-＂Yanglingquan＂ （GB 34） for 30 min, once daily for 2 t, 2 w, 3 w respectively. The paw withdrawal latency （PWL） was detected to be used as the pain threshold. Under deep anesthesia, the rat was decapitated for collecting blood samples. Plasma adrenocorticotropic hormone （ACTH）, β-endorphine （EP）, cortisone （COR） contents were detected using radioimmunoassay. Results： In comparison with control group, PWL difference values （PWLDV） of COl ＋ EA, OVX ＋ COl and OVX ＋COl ＋ EA groups increased significantly （P〈0. 05）, suggesting a marked decrease of pain threshold. Compared with COl group, PWLDV of COl ＋ EA group at 3 w decreased significantly （ P〈0. 05）, being close to normal control group （ P〉0. 05）. Compared with OVX＋ COl group, PWLDV of OVX＋ CCl ＋ EA at 2 w and 3 w lowered significantly （ P〈0. 05）, suggesting an evident pain relief. In comparison with CCl ＋ EA group, PWLDV of OVX＋ CCl ＋ EA group at most time courses were considerably higher （P〈0.05）, showing a better cumulative analgesic effect in COl rats without OVX.
出处
《针刺研究》
CAS
CSCD
2007年第5期306-312,共7页
Acupuncture Research
基金
国家自然科学基金资助项目(C03050309)
