摘要
采用聚乙烯醇和海藻酸钠双载体固定红曲菌细胞,以气升式生物反应器重复发酵对Lovastatin的培养条件进行了试验研究,结果表明,以20%粳米粉为基础培养基,添加葡萄糖和有机氮源能显著提高红曲菌M-01发酵Lovastatin产量;补加多种蔬菜浸出汁对红曲菌M-01产Lovastatin有明显地促进作用。气升反应器优化的培养条件为发酵培养基的初始pH4～5;最适发酵温度30℃;最适固定化细胞粒子的接入量20%。固定化细胞粒子经20批次重复发酵其凝胶粒子完好,固定化细胞产Lovastatin始终处于较高值,其产量为2.59mg/mL～2.68mg/mL,为游离细胞发酵产Lovastatin的10倍。
Monascus cell immobilized with sodium alginate and polyvinyl alcohol （PVA） were used to produce lovastatin in an airlift bioreactor. The nutritional requirement of Monascus for lovastatin formation was studied. The experiment results showed that the basic culture medium of 20% rice powder with addition of glucose and organic nitrogen source or multi-vegetable juice could promote lovastatin level of Monascus M-01. The optimal fermentation condition was determined as： initial pH 4-5, temperature 30℃, 20% inoculum quantity of immobilized cell particles. The micelle of immobilized cell remained monolithic and a high level of lovastatin was maintained during 20 times successive fermentation, lovastatin yield ranged between 2.59-2.68 mg/ml, ten times higher than that produced by free Monascus cell fermentation.
出处
《中国酿造》
CAS
北大核心
2007年第11期40-42,47,共4页
China Brewing
基金
教育部高等学校生物反应器工程国家重点实验室访问学者基金资助(F200-C-09)
