摘要
高铁血红蛋白(metHb)含量是影响血液代用品携氧-释氧性能的主要因素之一。以市售的牛血红蛋白(Hb)冻干粉(90%以上已氧化)为原料,采用强还原剂连二亚硫酸钠将metHb还原成亚铁状态,进一步采用溶剂扩散-挥发复乳化法制备载牛Hb聚合物型纳米微囊血液代用品。研究制备过程中不同工艺条件对微囊中metHb含量的影响,并对制备过程的工艺参数进行优化。结果表明,连二亚硫酸钠与牛Hb摩尔比为2:1时原料中metHb基本被还原。制备过程中较低的乳化强度(如初乳采用超声波输出功率27W作用12s、复乳采用10000r/min匀浆乳化1.5min),油相中水溶性溶剂丙酮的加入及嵌段了PEG链段的聚合物能有效抑制Hb氧化,通过优化工艺最终使微囊内metHb含量从原料的90%以上降为8.6%,使其具备良好的携氧-释氧性能。该方法是控制微囊型血液代用品中metHb含量,进而实现其高效携氧-释氧功能的理想途径。
The metHb content is one of the main factors affecting the oxygen carrying-releasing property of blood substituies. Bovine Hb in lyophilized form （with over 90% oxidized） purchased from market was used as raw materials. The powerful reductant sodium dithionite （SD） was employed to reduce the oxidized metHb to ferrous state, which was further encapsulated in polymeric nanoparticles blood substitues by the solvent diffusion-evaporation double emulsion method. The preparative conditions influencing the metHb content in nanoparticles were investigated and optimized. The results showed that the metHb in the raw bovine Hb could be reduced to ferrous state when the molar ratio of SD to Hb was 2： 1. Low emulsification strength in preparation process was 27W for 12s by ultrasonic and 10000r/min for 1.5min of homogenization during the primary and secondary emulsion, respectively. The addition of miscible solvents such as acetone and the biodegradable polymers with PEG segments in oil phase could suppress Hb oxidation. The resultant metHb level decreased from initial over 90% in the raw bovine Hb to 8.6%, which showed better capacity of carrying-releasing oxygen. The method presented here may provide an ideal way to control the metHb level in the Hb-loaded nanoparticles blood substitutes and realize the function of carrying-releasing oxygen effectively.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2007年第5期787-792,共6页
Chinese Journal of Biomedical Engineering
基金
十五"863"纳米专项(2004AA-302050)
上海市科委纳米专项(0452nm022)。
关键词
高铁血红蛋白
氧化
复乳
携氧-释氧
methemoglobin
oxidation
double emulsion
carrying-releasing oxygen
