摘要
目的:研究马桑内酯所致癫痫持续状态下海马结构内突触体素(SYN)及胶质原纤维酸性蛋白(GFAP)表达的变化规律,探讨癫痫的发生机制。方法:成年健康雄性SD大鼠30只,随机分为模型组及对照组,采用马桑内酯(50μg/kg)侧脑室注射法建立急性癫痫动物模型,应用免疫组织化学方法观察癫痫持续状态下SYN和GFAP的表达。结果:①模型组海马结构各区突触体素阳性免疫反应产物的密度较对照组普遍增加,尤以CA3区苔藓纤维层和齿状回分子层内带为甚(P<0.05)。②模型组海马结构内GFAP免疫反应阳性的胶质细胞与对照组相比,其平均光密度、胞体截面积和周长均显著增强或增大(P<0.05),GFAP免疫反应性增强。结论:突触体素表达的增强可能易化了神经递质的装载和释放,与癫痫持续状态的维持和加重相关。活化的星形胶质细胞在癫痫持续状态中可能主要起保护作用。
Objective： To study the changes in expressions of synaptophysin （SYN） and gliaI fibrillary acidic protein （GFAP） after coriaria lactone （CL） induced status epilepticus （SE）, and the mechanism of SE. Methods： 30 adull male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle （LV） with coriaria lactone （50μg/kg）. The sections of the brains were processed by immunochemistry with antibodies against SYN and GFAP. Results ： The density of synaptophysin positive immunoreactive product in hippocampal formation of model group was higher than the controls, especially in the mossy fiber layer of the area CA3 and the inner molecular layer in the dentate gyrus （P〈0.05）. Compared with control group, the average optical density and the sectional area of some of the GFAP positive astrocytes in hippocampal formation of model group were significantly increased （P〈0.05） and the immunoreactivity enhanced. Conclusion： The increase of the expression of SYN probably makes the loading and release of the neurotransmitter easier, which may by correlative to the sustained aggravation of the status epilepticus. Activated astrocyte probably acts a protective role in the status epilepticus.
出处
《陕西医学杂志》
CAS
北大核心
2007年第9期1118-1121,1145,共5页
Shaanxi Medical Journal
基金
四川省教育厅资助项目(No2002A065)
