摘要
[目的]研究马桑内酯所致急性癫痫动物模型中海马结构内突触体素表达的变化规律,探讨突触间神经递质的传递与癫痫持续状态之间的关系。[方法]成年健康雄性SD大鼠30只,随机分为模型组及对照组,采用马桑内酯(50μg/kg体重)侧脑室注射法建立急性癫痫动物模型,应用免疫组织化学方法观察癫痫持续状态下突触体素的表达。[结果]①突触体素免疫反应产物为棕黄色点状或颗粒状沉积,在海马结构内呈明显的板层样分布。②模型组海马结构各区突触体素阳性免疫反应产物的密度较对照组普遍增加,尤以CA3区苔藓纤维层和齿状回分子层内带为甚(P﹤0.05)。[结论]突触体素表达的增强可能易化了神经递质的装载和释放,与癫痫持续状态的维持和加重相关。
To study the changes in expression of synaptophysin（SYN） in hippocampal formation after coriaria lactone induced acute animal model of epilepsy,and the relationship between synaptic transmission and the mechanism of status epilepticus.[Methods]30 adult male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle with coriaria lactone（50 μg/kg）. The sections of the brains were processed by immunochemistry with antibodies against SYN.[Results]①The SYN immunostaining results were observed as yellow spots or granule-like deposits,which were characterized by a laminar distribution pattern in hippocampal formation of all model and control group rats. ②The density of synaptophysin positive immunoreactive product in hippocampal formation of model group was higher than the controls,especially in the mossy fiber layer of the area CA3 and the inner molecular layer of the dentate gyrus （P﹤0.05）.[Conclusion]The increase of the expression of SYN probably makes the loading and release of the neurotransmitter easier,which may be correlative to the sustained aggravation of the status epilepticus.
出处
《现代预防医学》
CAS
北大核心
2007年第21期4048-4049,4053,共3页
Modern Preventive Medicine
关键词
突触体素
癫痫
海马
大鼠
synaptophysin
epilepsy
hippocampus
rats
