摘要
制备了异硫氰酸罗丹明B（TRITC）荧光纳米颗粒，于pH6．0的磷酸盐缓冲溶液中此荧光纳米颗粒在558nm光的激发下，于586nm波长处发射荧光。铜（Ⅱ）的存在可使此荧光纳米颗粒溶液的荧光发生猝灭。借此，构建了一种检测微量铜的方法。在最佳试验条件下，该方法测定铜的线性范围为5．00×10^-5～3．50×10^-4mol·L^-1，其回归方程为F／F0=8．4252-1．009×10^-6c，检出限为3．55×10^-5mol·L^-1。
Isothiocyanato-rhodamine B doped fluorescent nanoparticles were prepared and the fluorescence characteristics were studied. As shown by experimental result, fluorescence emission at 586 nm was observed when aqueous solution of the fluorescent nanoparticles was excited at 558 nm. And the emitted fluorescence was found to be quenched by cupric ion, in a phosphate buffer solution of pH 6.0. Based on these facts, a fluorospectrophotometric method for the determination of traces of copper was proposed. Linear relationship was kept in the range of 5.00×10^-5～3.50×10^-4mol·L^-1Cu（Ⅱ ）, according to the linear regression equation F/F0=8.4252-1.009×10^-6cc. Detection limit （3S） was found to be 3.55×10^-5mol·L^-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2007年第10期805-807,810,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
上海市教委项目(05DZ01
05JG05054
06ZZ16)
化学生物传感与计量学国家重点实验室课题(2005001)
关键词
荧光光度法
荧光纳米颗粒
荧光猝灭效应
铜离子
Fluorospectrophotometry
Fluorescent nanoparticles
Fluorescence quenching effect
Cupric ion
