摘要
从发病的杂交鲍(皱纹盘鲍×盘鲍)稚鲍中共分离27株菌株,并对其分泌胞外产物(包括酪蛋白酶、明胶酶、淀粉酶、脂肪酶、磷脂酶和溶血素)的能力进行了分析。试验结果表明,具有分泌胞外蛋白酶、明胶酶、淀粉酶、脂肪酶、磷脂酶和溶血素能力的菌株分别占总菌株数的81.5%(22/27)、74.1%(20/27)、18.5%(5/27)、0%(0/27)、11.1%(3/27)、29.6%(8/27)。其中,1#、14#菌株具有很强的产胞外蛋白酶能力,而明胶酶的最大分泌者为2#、7#、9#、17#菌株,淀粉酶的为11#、14#菌株,磷脂酶的为11#、15#菌株,溶血素的为菌株10#、21#。此外,10#、11#、15#菌株可同时分泌4种胞外产物,而12#、14#、18#、21#、23#、24#菌株可同时分泌3种胞外产物。综合考虑每一种胞外酶的潜在致病作用,以及菌株分泌多种胞外酶的能力,初步确认11株菌为潜在的致病菌:1#、2#、7#、9#、10#、11#、12#、14#、15#、17#、21#。用API条带法对它们进行了种类鉴定,11株菌中巴斯德氏菌和假单胞菌所占的比例分别为45.5%、27.3%。
Twenty-seven bacterial strains were isolated from creeping diseased hybrid abalones（Haliotis discus hannai×Haliotis discus） and extra-cellular secretions including protease,gelatinase,amylase,lecithinase and haemolysis were screened to find out the potential pathogen（s） at Zhangpu,Fujian Province.Results showed that the bacterial strains excreted extra-cellular protease,gelatinase,amylase,lipase,and lecithinase and accounted for 81.5%（22/27）,74.1%（20/27）,18.5%（5/27）,0%（0/27）,11.1%（3/27） or 29.6%（8/27）,in which strains 1 and 14 displayed high abilities to produce extra-cellular protease,while strains 2,7,9 and 17 had high abilities to produce gelatinase.Strains 11 and 14 had high abilities to produce extra-cellular amylase,while strains 11 and 15 had high abilities to produce lecithinase and strains 10 and 21 had high abilities to produce haemolysis on goat blood agar.Also,it was found that strains 10,11 and 15 produced four extra-cellular products while strains 12,14,18,21,23 and 24 produced three extra-cellular products.Taking into consideration of the function of each extra-cellular product,11 out of 27 strains were considered as potential pathogens.API 20E identification of 11 strains showed that Pasteurella sp.and Pseudomonas sp.made up to 45.5% and 27.3% of the total 27 strains examined.In conclusion,Pasteurella sp.and Pseudomonas sp.were potentially the primary pathogens that caused the death of black abalones（H.nigra） juveniles.
出处
《水产科学》
CAS
北大核心
2007年第10期560-563,共4页
Fisheries Science
关键词
杂交鲍稚鲍
“漫爬病”
致病菌
胞外产物
hybrid abalone
creeping disease
pathogen
extra-cellular product
