摘要
目的:研究β-七叶皂苷钠对血管形成的作用及其作用机制。方法:采用大鼠主动脉片96孔板培养,检测β-七叶皂苷钠对体外血管形成的影响;采用MTT法研究β-七叶皂苷钠对ECV-304细胞增殖的影响;采用免疫组织化学法研究β-七叶皂苷钠对小鼠S180肉瘤内血管内皮生长因子(VEGF)表达的影响。结果:β-七叶皂苷钠50μg.mL-1组在d1～d5对大鼠主动脉片血管出芽发生率有明显的抑制作用,抑制率均>39.0%;在d2～d6大鼠主动脉片血管相对面积明显小于阴性对照组,抑制率均>68.9%。MTT实验证明β-七叶皂苷钠对ECV-304细胞增殖具有明显抑制作用:100μg.mL-1抑制率为70.3%,50μg.mL-1抑制率为53.6%;免疫组织化学实验β-七叶皂苷钠1.4和2.8mg.kg-1组VEGF表达量均明显少于生理氯化钠溶液对照组。结论:β-七叶皂苷钠具有抑制血管形成的作用,其发挥作用的可能机制是抑制血管发生、内皮细胞的增殖及VEGF的表达。
Objective： To investigate the antiangiogenic effect and possible mechanism of sodium aescinate. Methods： Rat aortic disks were cultured in 96-well plate to test if sodium aescinate could inhibit the initiation and growth of aortic disks＇ outgrowths. MTT method was used to test if sodium aescinate could affect the proliferation of ECV-304 cell. Immunohistochemistry was performe~ to test if sodium aescinate could affect the secretion of VEGF in S180 sarcoma. Results： Sodium aescinate（50μg·mL^-1） could obviously inhibit the initiation of rat aortic disks from the first to the fifth day, the inhibition rates were more than 39.0% ; and from the second to the sixth day, it could obviously inhibit the growth of aortic disks＇ outgrowths, the inhibition rates were all more than 68.9%. The proliferation of ECV-304 was obviously inhibited, the inhibition rate was 70.3% and 53.6% for 100 and 50μg·mL^-1, respectively, The secretion of VEGF in S180 sarcoma of mouse were obviously decreased by sodium aescinate at the dose of 1.4 and 2.8 mg·mL^-1, Conclusion ： Sodium aescinate has obvious antiangiogenic effect. The initiation of angiogenesis and proliferation of endothelial cell are inhibited and the secretion of VEGF is also decreased.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2007年第17期1357-1360,共4页
Chinese Journal of New Drugs
基金
国家十五攻关(863计划)项目(2004AA2Z3783)
