摘要
背景与目的:建立H22肝癌腹水瘤荷瘤小鼠模型,通过给予模型鼠平调饮灌胃评价其抗肿瘤作用。材料与方法:用H22腹水肝癌株种植于小鼠右腋皮下,致移植性肿瘤,建立H22肝癌腹水瘤荷瘤小鼠模型。将模型鼠分成阴性对照组,平调饮组,平调饮+5-Fu组和5-Fu组,共4组,各组模型鼠分别灌胃生理盐水、平调饮、平调饮+5-Fu和5-Fu,连续灌胃10d后,观察瘤体大小、胸腺指数、脾脏指数、抑瘤率、生命延长率和VEGF在肿瘤及肿瘤周围组织中的表达。结果:平调饮组肿瘤平均瘤体、VEGF值减小,胸腺指数、脾脏指数、抑瘤率(48.6%)及生命延长率(168.3%)增高。结论:平调饮可提高胸腺指数,降低5-Fu对免疫功能的毒性作用,显著延长荷瘤小鼠的生存期。平调饮可能通过促进肿瘤细胞分化成熟,抑制肿瘤细胞增殖,降低肿瘤组织和肿瘤周围组织中VEGF表达,而对H22腹水型小鼠肿瘤的发展起抑制作用。
BACKGROUND ＆ AIM： To establish a model of mouse hepatoma H22 and to study the effects of Pingtiaoyin on the tumor. MATERIALS AND METHODS： The model of transplanted tumor was made by vaccination of hepatoma H22 in the right axilla.The model mice were divided into 4 groups： 0.9% NaC1, Pingtiaoyin, Pingtiaoyin ＋ 5-Fu and 5-Fu.The diameter of transplanted tumor, inhibition rates, thymus and spleen indexes, the rates of life prolongation and the expression of VEGF in carcinoma and peri-carcinoma tissues were analyzed. RESULTS： The diameter of the tumor and the expression of VEGF in the carcinoma were reduced, thymus and spleen indexes, the inhibition rates and the life prolongation rates were increased after treatment with Pingtiaoyin. CONCLUSION： Pingtiaoyin could improve the thymus index, reduce the negative effect of 5-Fu on the immune function, and prolong the survival of the model mouse. Pingtiaoyin also could inhibit the growth of transplanted tumor in mouse through increasing the differentiation of carcinoma cells, reducing their proliferation and downregulating the expression of VEGF in carcinoma and pre-carcinoma tissues.
出处
《癌变．畸变．突变》
CAS
CSCD
2007年第5期392-394,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
