摘要
微生物法检测牛乳中抗生素残留。采用对抗生素敏感的嗜热链球菌为试验菌,通过检验样品能否导致培养物的颜色变化来确定牛乳中抗生素的残留情况。利用GB/T 4789.27-2003所述方法,分别确定牛乳中的青霉素钠、磺胺二甲基嘧啶、磺胺甲噁唑、磺胺间二甲氧嘧啶,结果表明,微生物法对青霉素钠较敏感,检出量达到0.007 U·mL-1,对磺胺类抗生素的反应很低,无法达到准确判断牛乳中是否存在超过限量的磺胺类抗生素。
Antibiotic residues in milk were detected by microbiological method. The analysis was based on the color change in culture induced by milk sample, in which Streptococcus thermophilus was sensitive to antibiotic. When GB/T 4789.27-2003 method was employed to analyze penicillin Na, sulfamethoxazole, sulfamerazinand sulfadimethoxine. The results showed that the method was sensitive to penicillin Na, with detection limit 0.007 U·mL^-1. However, the method was not sensitive to other three sulfonamides. The analysis result couldn＇t show if there existed sulfonamides in milk, even when the level of each sulfonamide was more than its maximal residue limit.
出处
《东北农业大学学报》
CAS
CSCD
2007年第4期507-510,共4页
Journal of Northeast Agricultural University
基金
十一五"国家奶业重大专项"东北农区奶业集约化生产技术集成及产业化示范子课题-原料奶及乳制品生产全过程质量与安全控制技术的研究"
黑龙江省十一五科技专项课题"乳及乳制品安全与全程质量控制
关键词
抗生素
牛乳
分析
微生物方法
antibiotics
milk
analysis
microbiological method
