摘要
目的:探索理想的冷冻人脂肪间充质干细胞的方法。方法:采用常规方法体外培养并扩增人脂肪间充质干细胞,消化细胞并洗涤离心加入10%二甲基亚砜、30%胎牛血清、60%MEM的细胞冻存体系,直接置-70℃冰箱贮存。冻存4、8和12周后,37℃水浴复温,通过检测细胞周期﹑细胞表型和成脂的多向分化潜能,以鉴定该方法的可行性。结果:消化后不经过传统的程序性降温冻存技术,直接在-70℃冰箱贮存,其细胞生物学特性无明显改变,细胞仍存在成脂的多向分化潜能。结论:人脂肪间充质干细胞悬液直接-70℃冰箱贮存不影响其生物学特性,是冷冻保存的一种可行方法。
Objective To study an ideal cryopreservation method of Human Bone Mesenchymal stem cells in vitro. Methods Mesenchymal stem cells from human adipose were isolated by standard method and characterized with their morphology. cell-surface antigen profile and differentiation repertoire in vitro.The culture-expanded Mesenchymal stem cells were cryopresered in situ with culture medium （containing 10%DMSO, 30% selected FCS and 60% MEM in -70℃. Fellowing recovery of cryopreservation, differentiation to adipocytes in vitro and cell cycle analysis were performed to investigate whether the cryopreservation would change the differentiation potential of human adipose mesenchymal stem cells. Results After recovery of cryopreservation, there was not any detected changes as compared with the culture expanded human adipose mesenchymal stem in both differentiation potency and growth pottern at 12 weeks. Conclusion This optimized short term in situ cryopreservation at -70℃ could retain biological characterizes of human adipose mesenchymal stem cells for at least 3 months,and this method may be useful for cryopreservation of human adipose mesenchymal stem cells.
出处
《中国美容医学》
CAS
2007年第8期1026-1028,共3页
Chinese Journal of Aesthetic Medicine
基金
全军"十一五"医学科研面上项目(06M079)
甘肃省自然科学基金(3ZS061-A25-099)
关键词
人脂肪间充质干细胞
分化
脂肪细胞
冷冻贮存
human adipose mesenchymal stem cells
differentiate
adipocytes
cryopreservation
