摘要
为了解１６－羟基雷公藤内酯醇（１６－ｈｙｄｒｏｘｙｔｒｉｐｔｏｌｉｄｅ，Ｌ２）单体抗生育作用，选用健康成年雄性大白鼠观察Ｌ２对其精子生成及对睾丸、附睾和内脏器官的影响。实验分为两个阶段。第一阶段分别饲以Ｌ２单体０．１２ｍｇ／ｋｇ／ｄ、０．０６ｍｇ／ｋｇ／ｄ及０．０３ｍｇ／ｋｇ／ｄ，每周６ｄ，连续４周。取材观察附睾精子活力及睾丸生精上皮，结果显示大剂量Ｌ２可抑制睾丸生精过程，导致生精上皮大范围损伤脱落；中剂量Ｌ２可抑制生精过程，但生精上皮损伤不明显；小剂量Ｌ２抑制生精过程不明显。第二阶段给大鼠饲服Ｌ２单体０．０６ｍｇ／ｋｇ／ｄ、每周６ｄ，连续８周。结果表明，实验组的精子活力为０，精子头部正常，琥珀酸脱氢酶反应阴性，睾丸内可见成熟精子减少，曲细精管内生精上皮明显损伤，有的管腔内上皮脱落，生精上皮缺失，并可见上皮细胞融合形成多核巨细胞。对照组及实验组大鼠肝、肾琥珀酸脱氢酶反应及形态学观察均未见明显异常。
The 16 Hydroxytriptolide (L2) is a new diterpenepoxide purified from Tripterygium wilfordii Hook.f.(TW). Our present study reported the antifertility effect of 16 Hydroxytriptolide (L2) on male rat. The results showed that L2, if the rats were fed with L2 at high concentration (0.12mg/kg/d) for 4w, could result in the spermatogenic cells falling off. However, if the dosages were used at moderate concentration (0.06mg/kg/d) for 4w, L2 could lead to male antifertility but did not damage the spermatogenic cells. If the dosages were used at low concentration (0.03mg/kg/d) for 4w, L2 had no effect on male rat antifertility. So, we choised the moderate dosage L2 (0.06mg/kg/d) for another experiment for 8w. L2 exerted very strong antifertility effect for all experimental rats, the sperm motility was zero, the sperm numbers were decreased and seminiferous tubule epithelium falling off or formed large multinuclear cells. However, L2 did not damage rat′s liver and kidney.
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第2期214-217,共4页
Acta Anatomica Sinica
基金
国家计划生育委员会"8.5"攻关课题资助
关键词
羟基
雷公藤内酯醇
抗生育药
生精细胞
药理
hydroxytriptolide(L2)
Antifertility effect
Seminiferous tubule
Rat
